Cytokeratin 19 Antibody (A-3): sc-376126

Cytokeratin 19 Antibody (A-3) is a mouse monoclonal IgG3 kappa light chain antibody developed to detect Cytokeratin 19, also known as KRT19, a pivotal intermediate filament protein in epithelial cells. Cytokeratin 19 plays a crucial role in maintaining the structural integrity and mechanical resilience of epithelial tissues, facilitating cell differentiation and tissue specialization. Located within the cytoskeletal network of the cell, Cytokeratin 19 interacts with other keratins such as KRT8 and binding proteins like plectin and desmoplakin, which are essential for linking the cytoskeleton to cellular junctions and ensuring cellular stability. Cytokeratin 19 undergoes post-translational modifications, including phosphorylation, which regulate filament assembly and disassembly, thereby influencing cellular signaling pathways and dynamics essential for processes like cell migration and division. Anti-Cytokeratin 19 antibody (A-3) targets an epitope between amino acids 7-29 at the N-terminus of Cytokeratin 19, ensuring reliable detection across multiple species including mouse, rat, human, equine, and porcine, and is compatible with various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Cytokeratin 19 is extensively used as a biomarker in cancer research, aiding in the diagnosis and characterization of malignancies such as breast, liver, and lung cancers due to its expression in tumor cells and its fragments like CYFRA 21-1 serving as important tumor markers. Cytokeratin 19 monoclonal antibody (A-3) facilitates the study of epithelial cell behavior in both normal and pathological conditions, advancing our understanding of epithelial biology and tumor progression. Cytokeratin 19 (A-3) antibody offers flexibility for diverse experimental protocols in both non-conjugated and various conjugated forms, ensuring precise and consistent results in research and diagnostic endeavors.

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Cytokeratin 19 Antibody (A-3) References:

1. Expression of cytokeratin 19 mRNA in human lung cancer cell lines.  |  Ueda, Y., et al. 1999. Int J Cancer. 81: 939-43. PMID: 10362142
2. Elevation of cytokeratin 19 fragment in serum in patients with hepatoma: its clinical significance.  |  Nagai, T., et al. 2001. Eur J Gastroenterol Hepatol. 13: 157-61. PMID: 11246615
3. CYFRA 21-1 is released in TNF-alpha-induced apoptosis in the hepatocellular carcinoma cell line HuH-7.  |  Wu, F., et al. 2002. Int J Oncol. 21: 441-5. PMID: 12118343
4. Markers for dysplasia of the upper aerodigestive tract. Suprabasal expression of PCNA, p53, and CK19 in alcohol-fixed, embedded tissue.  |  Coltrera, MD., et al. 1992. Am J Pathol. 141: 817-25. PMID: 1384338
5. Immunocytochemistry of cytokeratins in primary human liver tumors.  |  Van Eyken, P., et al. 1991. APMIS Suppl. 23: 77-85. PMID: 1715730
6. Systematic review and meta-analysis of cytokeratin 19-based one-step nucleic acid amplification versus histopathology for sentinel lymph node assessment in breast cancer.  |  Tiernan, JP., et al. 2014. Br J Surg. 101: 298-306. PMID: 24536007
7. MR Features Based on LI-RADS Ver. 2018 Correlated with Cytokeratin 19 Expression in Combined Hepatocellular Carcinoma-Cholangiocarcinoma.  |  Zhou, C., et al. 2021. J Hepatocell Carcinoma. 8: 975-983. PMID: 34458204
8. Association Between Cytokeratin 19-Specific IgG and Neutrophil Activation in Asthma.  |  Quoc, QL., et al. 2024. Allergy Asthma Immunol Res. 16: 353-371. PMID: 39155736
9. Cytokeratin expression in normal and (pre)malignant head and neck epithelia: an overview.  |  van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025
10. Serum and tissue distribution of a fragment of cytokeratin 19 (cyfra 21-1) in lung cancer patients.  |  Quillien, V., et al. 1995. Anticancer Res. 15: 2857-63. PMID: 8669879
11. Functional inactivation of p53 by antisense RNA induces invasive ability of lung carcinoma cells and downregulates cytokeratin synthesis.  |  Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
12. Cytokeratin expression, fibrillar organization, and subtle function in liver cells.  |  Marceau, N. and Loranger, A. 1995. Biochem Cell Biol. 73: 619-25. PMID: 8714681

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cytokeratin 19 Antibody (A-3) has 46 citations in a variety of scientific publications.

Primary antibodies like Anti-Cytokeratin 19 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cytokeratin 19 Antibody (A-3) Specifications

Alternate Product Name: KRT19

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 7-29

Epitope Target: Cytokeratin 19

Epitope Species: human

Product Reactivity/Detection: Cytokeratin 19

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 46 citations in various scientific publications

Available Conjugates/Forms for Cytokeratin 19 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin