Aurora A Antibody (D-2): sc-373856
- Aurora A Antibody (D-2) is a mouse monoclonal IgG1 κ Aurora A antibody, cited in 7 publications, provided at 200 µg/ml
- raised against a peptide mapping near the C-terminus of Aurora A of Xenopus laevis origin
- Aurora A Antibody (D-2) is recommended for detection of Aurora A of Xenopus and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-Aurora A Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Aurora A Antibody (D-2) for WB applications. These reagents are now offered in bundles with Aurora A Antibody (D-2) (see ordering information below).
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Aurora A Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Aurora A of Xenopus origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Aurora A (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Aurora A is a crucial serine/threonine kinase that plays a significant role in regulating the cell cycle, particularly during the transition from G2 phase to mitosis. Aurora A′s proper function is vital for accurate chromosome segregation and cell division, and dysregulation of Aurora A is often implicated in tumorigenesis. Aurora A is primarily located in centrosomes and spindle poles during mitosis, where Aurora A is essential for formation and stabilization of the mitotic spindle. This localization ensures chromosomes are evenly distributed to daughter cells, preventing aneuploidy, which can lead to cancer. Anti-Aurora A antibody (D-2) is an invaluable tool for researchers studying cell cycle dynamics and molecular mechanisms underlying cancer progression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Aurora A Antibody (D-2) References:
- pEg2 aurora-A kinase, histone H3 phosphorylation, and chromosome assembly in Xenopus egg extract. | Scrittori, L., et al. 2001. J Biol Chem. 276: 30002-10. PMID: 11402032
- Identification of a functional destruction box in the Xenopus laevis aurora-A kinase pEg2. | Arlot-Bonnemains, Y., et al. 2001. FEBS Lett. 508: 149-52. PMID: 11707286
- Cell-cycle-dependent regulation of human aurora A transcription is mediated by periodic repression of E4TF1. | Tanaka, M., et al. 2002. J Biol Chem. 277: 10719-26. PMID: 11790771
- Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules. | Giet, R., et al. 2002. J Cell Biol. 156: 437-51. PMID: 11827981
- Aurora-A overexpression reveals tetraploidization as a major route to centrosome amplification in p53-/- cells. | Meraldi, P., et al. 2002. EMBO J. 21: 483-92. PMID: 11847097
- Involvement of Aurora A kinase during meiosis I-II transition in Xenopus oocytes. | Castro, A., et al. 2003. J Biol Chem. 278: 2236-41. PMID: 12426316
- Identification of novel phosphorylation sites on Xenopus laevis Aurora A and analysis of phosphopeptide enrichment by immobilized metal-affinity chromatography. | Haydon, CE., et al. 2003. Mol Cell Proteomics. 2: 1055-67. PMID: 12885952
- Aurora A and mitotic commitment. | Prigent, C. and Giet, R. 2003. Cell. 114: 531-2. PMID: 13678575
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aurora A Antibody (D-2) | sc-373856 | 200 µg/ml | $322.00 | |||
Aurora A Antibody (D-2): m-IgG Fc BP-HRP Bundle | sc-527419 | 200 µg Ab; 10 µg BP | $361.00 | |||
Aurora A Antibody (D-2): m-IgG1 BP-HRP Bundle | sc-532792 | 200 µg Ab; 20 µg BP | $361.00 | |||
Aurora A Antibody (D-2) AC | sc-373856 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Aurora A Antibody (D-2) HRP | sc-373856 HRP | 200 µg/ml | $322.00 | |||
Aurora A Antibody (D-2) FITC | sc-373856 FITC | 200 µg/ml | $336.00 | |||
Aurora A Antibody (D-2) PE | sc-373856 PE | 200 µg/ml | $349.00 | |||
Aurora A Antibody (D-2) Alexa Fluor® 488 | sc-373856 AF488 | 200 µg/ml | $364.00 | |||
Aurora A Antibody (D-2) Alexa Fluor® 546 | sc-373856 AF546 | 200 µg/ml | $364.00 | |||
Aurora A Antibody (D-2) Alexa Fluor® 594 | sc-373856 AF594 | 200 µg/ml | $364.00 | |||
Aurora A Antibody (D-2) Alexa Fluor® 647 | sc-373856 AF647 | 200 µg/ml | $364.00 | |||
Aurora A Antibody (D-2) Alexa Fluor® 680 | sc-373856 AF680 | 200 µg/ml | $364.00 | |||
Aurora A Antibody (D-2) Alexa Fluor® 790 | sc-373856 AF790 | 200 µg/ml | $364.00 | |||
Aurora A (D-2) Neutralizing Peptide | sc-373856 P | 100 µg/0.5 ml | $69.00 |