Molecular structure of PNPP, Disodium Salt, CAS Number: 4264-83-9
PNPP, Disodium Salt (CAS 4264-83-9)
See product citations (21)
Alternate Names:
PNPP, Disodium Salt is also known as p-Nitrophenyl phosphate disodium hexahydrate.
Application:
PNPP, Disodium Salt is an alkaline phosphatase substrate used in ELISA applications while absorbing light at 405nm.
CAS Number:
4264-83-9
Purity:
≥99%
Molecular Weight:
371.14
Molecular Formula:
C6H4NO6P•2Na•6H2O
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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Description
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SDS & Certificate of Analysis
A colorimetric alkaline phosphatase soluble substrate, PNPP, Disodium Salt is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures. The reaction with PNPP, Disodium Salt produces a soluble end product that is yellow in color. PNPP, Disodium Salt Can be used to perform kinetic and endpoint determinations. PNPP, Disodium Salt is also used as a phosphorylating agent in nucleotide synthesis. PNPP, Disodium Salt is recommended for use with pNPP Substrate Buffer (sc-296099). Also available in solution form: p-Nitrophenyl Phosphate 50X solution (sc-358718).
PNPP, Disodium Salt (CAS 4264-83-9) References
- Enzyme immunoassays in diagnostic medicine. Theory and practice. | Voller, A., et al. 1976. Bull World Health Organ. 53: 55-65. PMID: 1085667
- Stability of an extreme halophilic alkaline phosphatase from Halobacterium salinarium in non-conventional medium. | Marhuenda-Egea, FC., et al. 2001. J Biotechnol. 87: 255-61. PMID: 11334667
- Mechanism of adaptation of an atypical alkaline p-nitrophenyl phosphatase from the archaeon Halobacterium salinarum at low-water environments. | Marhuenda-Egea, FC., et al. 2002. Biotechnol Bioeng. 78: 497-502. PMID: 12115118
- Functional approach to the catalytic site of the sarcoplasmic reticulum Ca(2+)-ATPase: binding and hydrolysis of ATP in the absence of Ca(2+). | Lax, A., et al. 2004. J Bioenerg Biomembr. 36: 265-73. PMID: 15337857
- Reverse micelles in organic solvents: a medium for the biotechnological use of extreme halophilic enzymes at low salt concentration. | Marhuenda-Egea, FC., et al. 2002. Archaea. 1: 105-11. PMID: 15803648
- Inhibitory effect of okadaic acid on the p-nitrophenyl phosphate phosphatase activity of protein phosphatases. | Takai, A. and Mieskes, G. 1991. Biochem J. 275 (Pt 1): 233-9. PMID: 1850239
- Enzyme immunoassay ELISA and EMIT. | Engvall, E. 1980. Methods Enzymol. 70: 419-39. PMID: 6999296
- Conidial alkaline phosphatase from Neurospora crassa. | Say, JC., et al. 1996. Phytochemistry. 41: 71-5. PMID: 8588877
- Dependence of divalent metal ions on phosphotransferase activity of osseous plate alkaline phosphatase. | Ciancaglini, P., et al. 1997. J Inorg Biochem. 66: 51-5. PMID: 9173100
Inhibitor of:
BPGM.Substrate of:
Chromogenic, and HRP and AP.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PNPP, Disodium Salt, 25 g | sc-3720 | 25 g | $89.00 | |||
PNPP, Disodium Salt, 250 g | sc-3720A | 250 g | $395.00 |