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Tris base (CAS 77-86-1)

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Application:A high quality buffer used in the range of physiological pH 7-9
CAS Number:77-86-1
Molecular Weight:121.14
Molecular Formula:C4H11NO3
Refer to Certificate of Analysis for lot specific data (including water content).
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Tris base sc-3715 500 g $27
Tris base sc-3715A 1 kg $45
Tris base sc-3715B 5 kg $195
Tris base sc-3715C 10 kg $390
Tris base, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE (sc-296645) and TBE (sc-296650) buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.
Technical Information
Appearance:Crystalline powder
Physical State:Solid
pH:useful pH range: 7.0 - 9.0
Solubility:Soluble in water (550 mg/ml), ethylene glycol (79.1 mg/ml), methanol (26 mg/ml), anhydrous ethanol (14.6 mg/ml), 95% ethanol (22 mg/ml), DMF (14 mg/ml), acetone (2 mg/ml), ethyl acetate (0.5 mg/ml), olive oil (0.4 mg/ml), chloroform (0.05 mg/ml), and alkaline solution.
Storage:Store at room temperature
Melting Point:168-172° C
Boiling Point:219-220° C at 10 mmHg
Density:1.35 g/cm3 at 20° C
Refractive Index:n20D 1.54 (Predicted)
pK Values:pKa: 8.2 (at 20° C), 8.06 (at 25° C), 7.72 (at 37° C)
Safety and Reference Information
WGK Germany:2
PubChem CID:6503
Merck Index:14: 9772
MDL Number:MFCD00004679
EC Number:201-064-4
Beilstein Registry:741883
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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1. Kallet, R.H., et al. 2000. Am. J. Respir. Crit. Care Med. 161: 1149-1153. PMID: 10764304
2. Desmarais, W.T., et al. 2002. Structure. 10: 1063-1072. PMID: 12176384
3. Ghalanbor, Z., et al. 2008. Protein Pept. Lett. 15: 212-214. PMID: 18289113
4. Sambrook, J. and Russell, D.W. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor, NY. CSHL Press.
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