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CBP Antibody (A-22): sc-369

 |  Datasheet

(Based on data analysis)

  • CBP Antibody (A-22) is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope mapping at the N-terminus of CBP of human origin
  • recommended for detection of CBP p265 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine and porcine
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-369 X, 200 µg/0.1 ml
  • blocking peptide, sc-369 P
  • See 288 citations for CBP Antibody (A-22)
 

See additional CREB Binding Protein Antibodies.

Also see CREB Binding Protein Inhibitors for functional analysis of cellular responses to CREB Binding Protein.


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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
CBP Antibody (A-22) sc-369 200 µg/ml $279
CBP (A-22) P sc-369 P
(peptide)
 100 µg/0.5 ml $61
CBP Antibody (A-22) X sc-369 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
CBP siRNA (h) sc-29244 10 µM $258
CBP siRNA (m) sc-29243 10 µM $258
CBP (h)-PR sc-29244-PR 10 µM, 20 µl $23
CBP (m)-PR sc-29243-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
CBP shRNA Plasmid (h) sc-29244-SH 20 µg $520
CBP shRNA Plasmid (m) sc-29243-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
CBP shRNA (h) Lentiviral Particles sc-29244-V 200 µl $625
CBP shRNA (m) Lentiviral Particles sc-29243-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
HeLa nuclear extract sc-2120 250 µg/0.05 ml $143
KNRK nuclear extract sc-2141 250 µg/0.05 ml $143
Jurkat nuclear extract sc-2132 250 µg/0.05 ml $143
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human CREBBP 1387 16p13.3 NM_004380 Q92793
 600140
Mouse Crebbp 12914 16 A1 P45481
 N/A
 

Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression, even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.

CBP Antibody (A-22) Product Citations
See how others have used CBP Antibody (A-22) and or CBP Antibody (A-22) conjugates.


287 total citations
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CBP Antibody (A-22) Data
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sc-369
CBP (A-22): sc-369. Western blot analysis of CBP expression in KNRK nuclear extract.
sc-369
ChIP analysis of transcription factor binding to the Interferon β promoter before (A) and six hours after (B) Sendai virus infection of HeLa cells. Antibodies tested included NFκB p65 (A): sc-109, ATF-2 (N-96): sc-6233, GCN5 (N-18): sc-6303, Pol II (H-224): sc-9001, TFIID (TBP)(SI-1): sc-273 and CBP (A-22): sc-369. Data kindly provided by G. Mosialos.
sc-369
ChIP analysis of coactivator recruitment on Cyclin D2 promoter in C2C12 cells treated with LiCl and serum. Antibodies tested include β-catenin (H-102): sc-7199, β-catenin (C-18): sc-1496, β-catenin (E-5): sc-7963, Tip60 (N-17): sc-5725, TRRAP (T-17): sc-5405, TRRAP (Y-18): sc-12375, TRRAP (F-20): sc-12376, TRRAP (H-300): sc-11411, CBP (A-22): sc-369, CBP (C-20): sc-583, CBP (451): sc-1211, CPB (C-1): sc-7300, p300 (H-272): sc-8981, p300 (N-15): sc-584 and p300 (C-20): sc-585. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.
sc-369
ChIP analysis of p52 promoter occupancy in murine p19 cells in response to activation by estradiol (E2). Antibodies tested include ERα (MC-20): sc-542, ERα (H-184): sc-7207, CBP (A-22): sc-369, CBP (C-1): sc-7300, p/CIP (F2): sc-5305, p/CIP (M-397): sc-9119, RIP140 (H-300): sc-8997 and HDAC3 (N-19): sc-11417. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Perissi et al., Cell 2004, 116: 511-526.
sc-369
ChIP analysis of cofactor occupancy dynamics on the ICAM1 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, Bcl-3 (C-14): sc-185, Bcl-3 (H-146): sc-13038, PCAF (C-16): sc-6300, PCAF (H-369): sc-8999, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
sc-369
ChIP analysis of cofactor occupancy dynamics on the IL-8 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211, p300 (C-20): sc-sc-585, p300 (N-15): sc-584, p300 (H-272): sc-8981. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
sc-369
ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included RORα (C-16): sc-6062 (B), TIP60 (N-17): sc-5725 (C), CBP (A-22): sc-369 and CBP (C-20): sc-583 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
sc-369
ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included RORα (C-16): sc-6062 (B), TIP60 (N-17): sc-5725 (C), CBP (A-22): sc-369 and CBP (C-20): sc-583 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
sc-369
ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included RORα (C-16): sc-6062 (B), TIP60 (N-17): sc-5725 (C), CBP (A-22): sc-369 and CBP (C-20): sc-583 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
sc-369
ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included RORα (C-16): sc-6062 (B), TIP60 (N-17): sc-5725 (C), CBP (A-22): sc-369 and CBP (C-20): sc-583 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
sc-369
ChIP analysis of recruitment of CBP, p300, PCA and BRG1 to the IL-2α (CD25) promoter in vivo. Antibodies tested include CBP (A-22): sc-369, p300 (N-15): sc-584, PCAF (H-369): sc-8999, and Brg-1 (H-88): sc-10768. CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- thymocytes or Rag-/- thymocytes expressing TCRβ. Data kindly provided by J. Imbert and reproduced from Yeh, J-H., et al., Nucleic Acids Research, 2002, 30: 1944-1951, with permission from Oxford University Press.
sc-369
CBP (A-22): sc-369. Western blot analysis of CBP expression in KNRK (A) and Jurkat (B) nuclear extracts.
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