siRNA Transfection Medium: sc-36868

siRNA Transfection Medium is a specialized culture medium optimized for the transfection of small interfering RNAs (siRNAs) into eukaryotic cells, a process critical for conducting gene silencing studies. This medium is formulated to enhance the efficiency of siRNA uptake by cells without the need for serum removal, which is often required in standard transfection protocols. The medium contains a balanced mixture of ions and buffers that protect siRNA molecules from degradation while maintaining the physiological conditions conducive to cell health and growth. By stabilizing the siRNA and facilitating its entry into cells, the medium ensures that the siRNA can effectively initiate the RNA interference (RNAi) pathway. Once inside the cell, siRNA molecules are incorporated into the RNA-induced silencing complex (RISC). The RISC then uses the siRNA as a template to recognize and cleave complementary mRNA, leading to targeted gene silencing. This specific downregulation of gene expression allows researchers to investigate the function of particular genes, analyze gene regulatory networks, and explore cellular responses to gene silencing. The siRNA Transfection Medium is therefore instrumental in the field of genetic research, providing a reliable and efficient tool for manipulating gene expression and studying its effects on various biological functions and pathways.