PBS: powder for 1 L of 10X

PBS: powder for 1 L of 10X (Phosphate buffered saline) is ready to use for preparation of 1 L of concentrated 10X PBS buffer. PBS buffer is ideal for maintaining a constant pH, and since it is isotonic and non-toxic to cells, it can be used in a wide variety of biological applications. PBS is frequently used as a wash and dilution buffer, and is also used to take a reference spectrum when measuring protein adsorption.

• Reconstitute powder in ddH20, bringing final volume to 1 L (10X). Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 10 L of 1X PBS buffer with a phosphate buffer concentration of 0.01M, 0.027M potassium chloride, 0.137M sodium chloride, and 1.76 mM potassium phosphate. The PBS solution pH will be approximately 7.4.
• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).
• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.

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