This product has been titrated for optimal performance. Recommended use is 20 uL per test (1x106 cells).
recommended for use with either a single or dual laser Flow Cytometer fitted with appropriate acquisition and analysis software
The flow cytometer must be equipped with a 488 nm laser and must be capable of detecting light scatter (forward and side) and three-color fluorescence with emission detectable in three ranges: 515-545 nm, 562-607 nm and >650 nm, and it must be able to threshold and discriminate using the >650 channel.
isotype matched negative control available that includes: rat IgG2a FITC/Armenian hamster IgG PE/rat IgG PE-Cy5, sc-3618 CON
