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HDAC8 siRNA (h), shRNA and Lentiviral Particle Gene Silencers

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See HDAC8 Antibodies for analysis of cellular responses to HDAC8 gene silencers, including HDAC8 siRNA, HDAC8 shRNA Plasmids and HDAC8 shRNA Lentiviral Particles.

Also see chemical HDAC8 Inhibitors for functional analysis of cellular responses to HDAC8.


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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
HDAC8 siRNA (h) sc-35548 10 µM $258
HDAC8 (h)-PR sc-35548-PR 10 µM, 20 µl $23
HDAC8 shRNA Plasmid (h) sc-35548-SH 20 µg $520
HDAC8 shRNA (h) Lentiviral Particles sc-35548-V 200 µl $625

HDAC8 siRNA (h): sc-35548

 |  siRNA Datasheet / Protocol
  • 10 µM, 50-100 transfections
  • siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
  • for independent verification of HDAC8 gene silencing results, individual siRNA duplex components are also available upon request
  • suitable control antibody: HDAC8 (E-5): sc-17778
  • sqRT-PCR Primer also available: HDAC8 (h)-PR: sc-35548-PR, 20 µl
  • For further details describing the si/shRNA Gene Silencers click here
  • See 1 citations for HDAC8 siRNA (h)
 
 Recommended siRNA Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
siRNA Dilution Buffer sc-29527 1.5 ml $5
siRNA Transfection Reagent sc-29528 0.3 ml $142
siRNA Transfection Medium sc-36868 20 ml $9
siRNA Reagent System sc-45064 1 kit $331
Control siRNA-A sc-37007 10 µM $77
Control siRNA (FITC Conjugate)-A sc-36869 10 µM $104
HDAC8 shRNA Plasmid (h): sc-35548-SH shRNA Datasheet / Protocol
  • 20 µg, up to 20 transfections
  • shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
  • shRNA sequences correspond to HDAC8 siRNA Gene Silencer sequences
  • provided as transfection-ready purified plasmid DNA target-specific gene silencing
  • after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
  • shRNA Plasmids are intended only for non-commercial use. Click here for details.
  • suitable control antibody: HDAC8 (E-5): sc-17778
  • sqRT-PCR Primer also available: HDAC8 (h)-PR: sc-35548-PR, 20 µl
 Recommended shRNA Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Plasmid Transfection Reagent sc-108061 0.2 ml $71
Plasmid Transfection Medium sc-108062 20 ml $8
Control shRNA Plasmid-A sc-108060 20 µg $83
Control shRNA Plasmid-B sc-108065 20 µg $83
Control shRNA Plasmid-C sc-108066 20 µg $83
copGFP Control Plasmid sc-108083 20 µg $83
Puromycin dihydrochloride sc-108071 25 mg $30
 
HDAC8 shRNA (h)
Lentiviral Particles: sc-35548-V
Lentiviral Particles Datasheet / Protocol
  • 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
  • Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
  • Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
  • shRNA sequences correspond to HDAC8 siRNA siRNA Gene Silencer sequences
  • After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
  • Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
  • Lentiviral Particles are intended for non-commercial use. Click here for details.
 Recommended Lentiviral Particle Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Control shRNA Lentiviral Particles-A sc-108080 200 µl $176
copGFP Control Lentiviral Particles sc-108084 200 µl $176
Puromycin dihydrochloride sc-108071 25 mg $30
Polybrene® sc-134220 10 mg/ml $18
 Recommended Control Antibody (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
HDAC8 Antibody (E-5) sc-17778 200 µg/ml $279
goat anti-mouse IgG-HRP sc-2005 200 µg/0.5 ml $70
 
Recommended Support Products:
(click button of application of choice)
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human HDAC8 55869 Xq13.1 NM_018486 Q9BY41
300269
 
HDAC8 siRNA (h) Product Citations
See how others have used HDAC8 siRNA (h).


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HDAC8 siRNA (h) Data
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HDAC8 siRNA (h): sc-35548. Western blot analysis of HDAC8 expression in non-transfected control (A) and HDAC8 siRNA transfected (B) HeLa cells. Blot probed with HDAC8 (E-5): sc-17778. β Tubulin (D-10): sc-5274 used as specificity and loading control.
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