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Erg-1/2/3 (C-20) Antibody: sc-353 |
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- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping at the C-terminus of Erg-1 of human origin
- recommended for detection of Erg-1, Erg-2 and Erg-3 of mouse, rat and human origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-353 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-353 X, 200 µg/0.1 ml
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Erg-1/2/3 Background Information
Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element. Erg genes encode for multiple proteins due to alternative splicing and alternative usage of initiation codons.
| Erg-1/2/3 (C-20) Product Citations |
See how others have used Erg-1/2/3 (C-20): sc-353 antibody and or Erg-1/2/3 (C-20) antibody conjugates.
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Erg-1/2/3 (C-20)
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Erg-1/2/3 (C-20): sc-353. Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.
Erg-1/2/3 (C-20): sc-353. Western blot analysis of Erg-1/2/3 expression in Jurkat (A) and Jurkat + PMA (B) nuclear extracts.
Erg-1/2/3 (C-20): sc-353. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Erg-1/2/3 (C-20), followed by FITC-conjugated goat anti-rabbit IgG: sc-2012. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. Erg-1/2/3 (C-20): sc-353. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Erg-1/2/3 (C-20), followed by PE-conjugated goat anti-rabbit IgG: sc-3739. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. Erg-1/2/3 (C-20): sc-353. Western blot analysis of Erg-1/2/3 expression in KNRK nuclear extract.
Erg-1/2/3 (C-20): sc-353. Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing nuclear and cytoplasmic staining of cells in red pulp at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Erg-1/2/3 (C-20): sc-353. Western blot analysis of Erg-1/2/3 expression in non-transfected 293T: sc-117752 (A), human Erg-1/2/3 transfected 293T: sc-115805 (B) and Jurkat (C) whole cell lysates. Erg-1/2/3 (C-20): sc-353. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Erg-1/2/3 (C-20), followed by PE-conjugated chicken anti-rabbit IgG: sc-3762. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. |
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