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home » Transcription Regulators » E Antibody / E Antibodies » E2A.E12 (V-18): sc-349

E2A.E12 (V-18) Antibody: sc-349

  |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml
  • epitope mapping at the C-terminus of E2A.E12 of human origin
  • recommended for detection of E12 and E47 of mouse, rat and human origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG; also reactive with additional species, including equine, canine and bovine
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-349 X, 200 µg/0.1 ml
  • blocking peptide, sc-349 P
 
Additional E Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   FCM   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human TCF3 6929 19p13.3 NM_003200 P15923
 147141
Mouse Tcfe2a 21423 10 C1 P15806
 N/A
 
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
E2A.E12 (V-18) sc-349 200 µg/ml $279
E2A.E12 (V-18) P sc-349 P
(peptide)
 100 µg/0.5 ml $61
E2A.E12 (V-18) X sc-349 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
E2A siRNA (h) sc-35245 10 µM $258
E2A siRNA (m) sc-35246 10 µM $258
E2A siRNA (h2) sc-63307 10 µM $258
E2A (h)-PR sc-35245-PR 10 µM $23
E2A (m)-PR sc-35246-PR 10 µM $23
E2A (h2)-PR sc-63307-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
E2A shRNA Plasmid (h) sc-35245-SH 20 µg $520
E2A shRNA Plasmid (m) sc-35246-SH 20 µg $520
E2A shRNA Plasmid (h2) sc-63307-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
E2A shRNA (h) Lentiviral Particles sc-35245-V 200 µl $625
E2A shRNA (m) Lentiviral Particles sc-35246-V 200 µl $625
E2A shRNA (h2) Lentiviral Particles sc-63307-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
E2A (m): 293 Lysate sc-111268 100µg/200µl $205
E2A (m): 293T Lysate sc-119880 100µg/200µl $205
E2A (m2): 293T Lysate sc-119881 100µg/200µl $205
 WB Control Recombinant Proteins (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
E12 (208-649) sc-4082 50 µg $167
E47 (1-584) sc-4083 50 µg $167
E47 (1-584) sc-4083 WB 10 µg/0.1 ml $88

E2A.E12 Background Information
Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes MyoD, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). MyoD transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. MyoD-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. Both homo- and hetero-oligomers of these proteins are able to distinguish very closely related E box proteins and are believed to play important roles in lineage specific gene expression.

E2A.E12 (V-18) Product Citations
See how others have used E2A.E12 (V-18): sc-349 antibody and or E2A.E12 (V-18) antibody conjugates.


29 total citations
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E2A.E12 (V-18)
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sc-349
E2A.E12 (V-18): sc-349. Western blot analysis of E12/E47 expression in Jurkat (A) and K-562 (B) whole cell lysates.
sc-349
E2A.E12 (V-18): sc-349. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
sc-349
Western blot analysis of human recombinant E47 (A,B,D) and human recombinant E12 (C) fusion proteins. Antibodies tested include E47 (N-649): sc-763 (A), E2A (Yae): sc-416 (B) and E2A.E12 (V-18): sc-349 (C,D).
sc-349
E2A.E12 (V-18): sc-349. Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with E2A.E12 (V-18), followed by FITC-conjugated goat anti-rabbit IgG: sc-2012. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
sc-349
E2A.E12 (V-18): sc-349. Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with E2A.E12 (V-18), followed by PE-conjugated goat anti-rabbit IgG: sc-3739. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
sc-349
E2A.E12 (V-18): sc-349. Western blot analysis of E2A expression in non-transfected: sc-110760 (A) and mouse E2A transfected: sc-111268 (B) 293 whole cell lysates.
sc-349
E2A.E12 (V-18): sc-349. Western blot analysis of E2A expression in non-transfected: sc-110760 (A) and mouse E2A transfected: sc-111268 (B) 293 whole cell lysates.
sc-349
E2A.E12 (V-18): sc-349. Western blot analysis of E2A expression in non-transfected 293T: sc-117752 (A), mouse E2A transfected 293T: sc-119880 (B) and Jurkat (C) whole cell lysates.
sc-349
E2A.E12 (V-18): sc-349. Western blot analysis of E2A expression in non-transfected 293T: sc-117752 (A), mouse E2A transfected 293T: sc-119881 (B) and Jurkat (C) whole cell lysates.
sc-349
E2A.E12 (V-18): sc-349. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing nuclear staining of reaction center cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
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