epitope corresponding to phosphorylated Tyr 1349 of Met of human origin
recommended for detection of Tyr 1349 phosphorylated Met of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine and porcine
p-Met Background Information The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity.Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines. Tyrosine phosphorylation enhances the receptor kinase activity, while serine phosphorylation of Met on residue 985 has an inhibitory effect. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
p-Met (Tyr 1349)
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p-Met (Tyr 1349): sc-34086. Western blot analysis of Met phosphorylation in untreated (A) and EGF treated (B) A-431 whole cell lysates.
p-Met (Tyr 1349): sc-34086. Western blot analysis of Met phosphorylation in untreated (A) and EGF treated (B) HeLa whole cell lysates.
