p-nestin (Thr 1583) Antibody: sc-33880

nestin Background Information
Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. Nestin is also a component of the dynamic IF network during muscle development where it polymerizes with desmin and vimentin. Nestin co-assembles with vimentin or a-internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nm IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells, and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis. Thr 316 is a cdc2-specific phosphorylation site in Nestin. Phosphorylation at this site is increased during mitosis and is used as a marker for cdk5-specific phosphorylation. Cdk5 and cdc2 induces phosphorylation at both residues Thr 316 and Thr 1583.