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immunofluorescence image from recommended Nanog (1E6C4) monoclonal antibody replacement. Image 137486
This polyclonal antibody has been discontinued. See our recommended Nanog (1E6C4) monoclonal antibody (sample data shown).
immunofluorescence image from recommended Nanog (1E6C4) monoclonal antibody replacement. Image 137486
This polyclonal antibody has been discontinued. See our recommended Nanog (1E6C4) monoclonal antibody (sample data shown).
This polyclonal antibody has been discontinued. See our recommended Nanog (1E6C4) monoclonal antibody (sample data shown).

Nanog Antibody (H-155): sc-33759

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  • Nanog Antibody (H-155) is a rabbit polyclonal IgG; 200 µg/ml
  • epitope corresponding to amino acids 151-305 mapping at the C-terminus of Nanog of human origin
  • Discontinued polyclonal antibody

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Description

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For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

Ordering Information

Nanog (1E6C4): sc-293121 [ Recommended monoclonal replacement for Nanog (H-155) ]

Product NameCatalog #UNITPriceQtyFAVORITES

Nanog Antibody (1E6C4)

sc-293121
200 µg/ml
$316.00

Nanog Antibody (1E6C4): m-IgG Fc BP-HRP Bundle

sc-527320
200 µg Ab; 10 µg BP
$354.00

Nanog Antibody (1E6C4): m-IgG1 BP-HRP Bundle

sc-532693
200 µg Ab; 20 µg BP
$354.00

Nanog Antibody (1E6C4) AC

sc-293121 AC
500 µg/ml, 25% agarose
$416.00

Nanog Antibody (1E6C4) HRP

sc-293121 HRP
200 µg/ml
$316.00

Nanog Antibody (1E6C4) FITC

sc-293121 FITC
200 µg/ml
$330.00

Nanog Antibody (1E6C4) PE

sc-293121 PE
200 µg/ml
$343.00

Nanog Antibody (1E6C4) Alexa Fluor® 488

sc-293121 AF488
200 µg/ml
$357.00

Nanog Antibody (1E6C4) Alexa Fluor® 546

sc-293121 AF546
200 µg/ml
$357.00

Nanog Antibody (1E6C4) Alexa Fluor® 594

sc-293121 AF594
200 µg/ml
$357.00

Nanog Antibody (1E6C4) Alexa Fluor® 647

sc-293121 AF647
200 µg/ml
$357.00

Nanog Antibody (1E6C4) Alexa Fluor® 680

sc-293121 AF680
200 µg/ml
$357.00

Nanog Antibody (1E6C4) Alexa Fluor® 790

sc-293121 AF790
200 µg/ml
$357.00

I have an old vial of this antibody in my lab and would like to test if it is still suitable for western blotting, could you give me a recommended starting dilution? Many thanks

Asked by: bede
Thank you for your question. We recommend using this rabbit polyclonal antibody, Nanog (H-155): sc-33759, at a starting dilution of 1:200 for Western blot (range of 1:100-1:1,000).
Answered by: Technical Support
Date published: 2017-07-12
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Rated 1 out of 5 by from Did not work for us in immunofluorescenceDid not work for us in immunofluorescence applications (1:100) on human pluripotent stem cells despite numerous tries. We replaced it with sc-293121 which worked great.
Date published: 2015-12-18
Rated 4 out of 5 by from Antibody good for immunofluorescence withAntibody good for immunofluorescence with Human Dermal Fibroblasts.
Date published: 2015-12-01
Rated 4 out of 5 by from Various publishable data in IHC using humanVarious publishable data in IHC using human hESC and 293T cells. -SCBT Publication Review
Date published: 2015-02-24
Rated 3 out of 5 by from Robust band seen in WB using human recombinantRobust band seen in WB using human recombinant Nanog fusion protein. -SCBT QC
Date published: 2015-02-12
Rated 4 out of 5 by from Publishable data in WB using mouse MEF cellsPublishable data in WB using mouse MEF cells -SCBT Publication Review
Date published: 2015-02-07
Rated 4 out of 5 by from Various publishable data in IF using humanVarious publishable data in IF using human human embryonic stem cell (hESC) line WA01 (H1), Mesenchymal Stem Cells, Namalwa and WI-L2-NS cell lines. -SCBT Publication Review
Date published: 2015-01-10
Rated 3 out of 5 by from Publishable data in ChIP using mouse PCaPublishable data in ChIP using mouse PCa cells (prostate cancer cell). -SCBT Publication Review
Date published: 2015-01-06
Rated 3 out of 5 by from Various publishable data in WB using humanVarious publishable data in WB using human MSCs and Adipose-derived stem cells. -SCBT Publication Review
Date published: 2014-04-17
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Nanog Antibody (H-155) is rated 3.3 out of 5 by 8.
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