DAF-16 (ce-300) Antibody: sc-33738

DAF-16 Background Information
Several proteins involved in regulating the aging process in C. elegans have been identified. DAF-2, DAF-16 and AGE-1 (also known as DAF-23) regulate lifespan via an insulin-signaling pathway (1-3). In specific, decreases in DAF-2 signaling induce metabolic and developmental changes, as in mammalian metabolic control by the insulin receptor (1). DAF-16 encodes a member of the hepatocyte nuclear factor 3 (HNF-3)/forkhead family of transcriptional regulators (2). In humans HNF-3 activity is antagonized by insulin, causing the down regulation of developmental genes, raising the possibility that aspects of the DAF-16 regulatory system have been conserved (2). The gene AGE-1 encodes a homologue of mammalian phosphatidylinositol-3-OH kinase (PI(3)K) catalytic subunits and is required for non-dauer development and normal senescence (3). CLK-1, a homolog of the yeast COQ7/CAT5 protein, is thought to exert its effects on longevity via the synthesis of ubiquinone, an essential component of electron transport (4-7).