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home » Transcription Regulators » SRF Antibody / SRF Antibodies » SRF (G-20): sc-335

SRF (G-20) Antibody: sc-335

  |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml
  • epitope mapping within the C-terminus of SRF of human origin
  • recommended for detection of SRF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine, bovine and porcine
  • blocking peptide, sc-335 P
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-335 X, 200 µg/0.1 ml
 
Additional SRF Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human SRF 6722 6p21.1 NM_003131 P11831
 600589
Mouse Srf 20807 17 C Q9JM73
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
SRF (G-20) sc-335 200 µg/ml $279
SRF (G-20) P sc-335 P
(peptide)
 100 µg/0.5 ml $61
SRF (G-20) X sc-335 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
SRF siRNA (h) sc-36563 10 µM $258
SRF siRNA (m) sc-36564 10 µM $258
SRF siRNA (r) sc-156005 10 µM $258
SRF (h)-PR sc-36563-PR 10 µM $23
SRF (m)-PR sc-36564-PR 10 µM $23
SRF (r)-PR sc-156005-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
SRF shRNA Plasmid (h) sc-36563-SH 20 µg $520
SRF shRNA Plasmid (m) sc-36564-SH 20 µg $520
SRF shRNA Plasmid (r) sc-156005-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
SRF shRNA (h) Lentiviral Particles sc-36563-V 200 µl $625
SRF shRNA (m) Lentiviral Particles sc-36564-V 200 µl $625
SRF shRNA (r) Lentiviral Particles sc-156005-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
Jurkat nuclear extract sc-2132 1000 µg $143
K-562 nuclear extract sc-2130 1000 µg $143
MCF7 Whole Cell Lysate sc-2206 500 µg/200 µl $104

SRF Background Information
Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1) have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.

SRF (G-20) Product Citations
See how others have used SRF (G-20): sc-335 antibody and or SRF (G-20) antibody conjugates.


62 total citations
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SRF (G-20)
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sc-335
SRF (G-20): sc-335. Western blot analysis of SRF expression in Jurkat nuclear extract.
sc-335
SRF (G-20): sc-335. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human spleen tissue showing nuclear staining.
sc-335
SRF (G-20): sc-335. Western blot analysis of SRF expression in Jurkat whole cell lysate (A) and Jurkat whole cell lysate immunoprecipitated with SRF (A-11): sc-25290 and detected with SRF (G-20): sc-335 (B).
sc-335
SRF (G-20): sc-335. ChIP analysis of SRF recruitment to human genomic CYR61 (1), ERG1 (2), ERG2 (3) , FOS (4) and two control amplicons (6,7) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).
sc-335
SRF (G-20): sc-335. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing nuclear staining of follicle and non follicle cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
sc-335
SRF (G-20): sc-335. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
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