epitope mapping at the N-terminus of IκB-ζ of human origin
recommended for detection of IκB-ζ of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including bovine and porcine
IκB-ζ Background Information I˚B-Ω (also called MAIL-S or INAP) is a member of the I˚B family. It shares a 30% identity with other family members and consists of six ankyrin repeats at its C-terminal. I˚B-Ω accumulates in the nucleus and, in humans, associates with the p50 and p65 subunits of nuclear NF˚B via its ankyrin repeats. The mouse homologue of I˚B-Ω has only been shown to associate with the p50 subunit. I˚B-Ω inhibits DNA binding and activity of the transcription factor NF˚B. Distinct from other I˚B family members, I˚B-Ω is not degraded upon cell stimulation and activation of NF˚B, rather evidence shows that it is up-regulated under these circumstances. This suggests that I˚B-Ω plays a significant role in regulation of NF˚B and that NF˚B may regulate I˚B-Ω in a negative feedback loop. Regulation of NF˚B by I˚B-Ω may differ depending on the species.
