p-Ac-Histone H3 (Ser 11/Lys 115) Antibody sc-33361

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p-Ac-Histone H3 (Ser 11/Lys 115) Antibody: sc-33361	\| Datasheet

rabbit polyclonal IgG, 200 µg/ml
epitope corresponding to dually phosphorylated Ser 11 and aceylated Lys 15 of Histone H3 of human origin
recommended for detection of Ser 11 phosphorylated and Lys 15 acetylated Histone H3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
blocking peptide, sc-33361 P

Additional Histone Antibodies ...

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WB IP IF siRNA

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

Human	HIST1H3D	8351	6p22.1	NM_003530	NP_003521	602811
Mouse	Hist2h3c1	15077	3 F2.1		P84228	N/A
Mouse	Hist2h3c2	97114	3 F2.1		P84228	N/A
Mouse	Hist1h3g	97908	13 A3.1		P68433	N/A
Mouse	Hist1h3f	260423	13 A3.1		P84228	N/A
Mouse	Hist1h3c	319148	13 A3.1		P84228	N/A
Mouse	Hist1h3d	319149	13 A3.1		P84228	N/A
Mouse	Hist1h3b	319150	13 A3.1		P84228	N/A
Mouse	Hist1h3e	319151	13 A3.1		P84228	N/A
Mouse	Hist1h3h	319152	13 A3.1		P68433	N/A
Mouse	Hist1h3i	319153	13 A3.1		P68433	N/A
Mouse	Hist2h3b	319154	3 F2.1		P84228	N/A
Mouse	Hist1h3a	360198	13 A3.1		P68433	N/A

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Ordering Information

Product Name	Catalog #	Unit	Price
p-Ac-Histone H3 (Ser 11/Lys 115)	sc-33361	200 µg/ml	$279
p-Ac-Histone H3 (Ser 11/Lys 115) P	sc-33361 P (peptide)	100 µg/0.5 ml	$61
Lambda Phosphatase	sc-200312	100,000 units	$462
Lambda Phosphatase	sc-200312A	20,000 units	$116

WB Positive Control Cell Lysates (click product name for more information)

Product Name	Catalog #	Unit	Price
NIH/3T3 Whole Cell Lysate	sc-2210	500 µg/200 µl	$104
Histone cluster 1 H3E (h): 293T Lysate	sc-116365	100µg/200µl	$205
Histone H3.3B (m): 293T Lysate	sc-125446	100µg/200µl	$205
Histone H3.3A (h3): 293T Lysate	sc-159580	100µg/200µl	$205
HeLa Whole Cell Lysate	sc-2200	500 µg/200 µl	$104

p-Ac-Histone H3 Background Information
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of Histone H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at Lysine 14 and 18 and Histone H4 at Lysine 5 and 8. Histone H4 may also be acetylated at Lysine 12 and 16, and the involvement of acetylated H4 with Histone H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling. Phosphorylation of Histone H3 is involved in chromosome condensation during mitosis. Histone H3 phosphorylation correlates to the expression of immediate-early genes such as c-Jun, c-Fos and c-Myc. Research indicates that MSK1, a growth and stress activated kinase, also phosphorylates Histone H3 in vitro.

p-Ac-Histone H3 (Ser 11/Lys 115)

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p-Ac-Histone H3 (Ser 11/Lys 15): sc-33361. Western blot analysis of phosphorylated Ac-Histone H3 expression in 293 (A) and HeLa (B) whole cell lysates.

p-Ac-Histone H3 (Ser 11/Lys 15): sc-33361. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

p-Ac-Histone H3 (Ser 11/Lys 15): sc-33361. Western blot analysis of Histone cluster 1 H3E phosphorylation in non-transfected 293T: sc-117752 (A), human Histone cluster 1 H3E transfected 293T: sc-116365 (B) and NIH/3T3 (C) whole cell lysates.

p-Ac-Histone H3 (Ser 11/Lys 15): sc-33361. Western blot analysis of Histone H3.3B phosphorylation in non-transfected 293T: sc-117752 (A), mouse Histone H3.3B transfected 293T: sc-125446 (B) and HeLa (C) whole cell lysates.

p-Ac-Histone H3 (Ser 11/Lys 15): sc-33361. Western blot analysis of Histone Ac-H3.3A phosphorylation in non-transfected 293T: sc-117752 (A), human Histone H3.3A transfected 293T: sc-159580 (B) and NTERA-2 cl.D1 (C) whole cell lysates.

Western blot analysis of Histone H3 phosphorlation and acetylation in untreated (A,H), Trichostatin A treated (B,I), calyculin A treated (C,J), Trichostatin A and calyculin A treated (D,K), Trichostatin A and lambda protein phosphatase treated (E,L), calyculin A and lambda protein phosphatase treated (F,M) and Trichostatin A, calyculin A and lambda protein phosphatase treated (G,N) NIH/3T3 whole cell lysates. Antibodies tested include p-Ac-Histone H3 (Ser 11/Lys 15): sc-33361 (A,B,C,D,E,F,G) and Histone H3 (FL-136): sc-10809 (H,I,J,K,L,M,N).

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p-Ac-Histone H3 (Ser 11/Lys 115) Antibody: sc-33361