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TIF1β Antibody (H-300): sc-33186

 |  Datasheet

(Based on data analysis)

  • TIF1β Antibody (H-300) is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope corresponding to amino acids 536-835 mapping at the C-terminus of TIF1β of human origin
  • recommended for detection of TIF1β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-33186 X, 200 µg/0.1 ml
  • See 3 citations for TIF1β Antibody (H-300)
 
Full-length TIF1β protein sequence with immunogen highlighted:
MAASAAAASAAAASAASGSPGPGEGSAGGEKRSTAPSAAASASASAAASSPAGGGAEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPAAPAAANSSGDGGAAGDGTVVDCPVCKQQCFSKDIVENYFMRDSGSKAATDAQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKSTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGMAIVKEEETEAAIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP

See additional TIF1 Antibodies including TIF1, TIF1 alpha, TIF1 beta and TIF1 gamma.

Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
TIF1β Antibody (H-300) sc-33186 200 µg/ml $279
TIF1β Antibody (H-300) X sc-33186 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
TIF1β siRNA (h) sc-38550 10 µM $258
TIF1β siRNA (m) sc-38551 10 µM $258
TIF1β siRNA (h) sc-38550-PR 10 µM, 20 µl $23
TIF1β (m)-PR sc-38551-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
TIF1β shRNA Plasmid (h) sc-38550-SH 20 µg $520
TIF1β shRNA Plasmid (m) sc-38551-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
TIF1β shRNA (h) Lentiviral Particles sc-38550-V 200 µl $625
TIF1β shRNA (m) Lentiviral Particles sc-38551-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
CCRF-CEM nuclear extract sc-2146 250 µg/0.05 ml $143
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
TIF1β (h): 293T Lysate sc-116358 100 µg/200 µl $205
CCRF-CEM Cell Lysate sc-2225 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human TRIM28 10155 19q13.43 NM_005762 Q13263
 601742
Mouse Trim28 21849 7 A1 Q62318
 N/A
 

TIF1∫, for transcriptional intermediary factor 1-beta, also designated KAP1(for KRAB-associated protein 1), TF1∫ and TRIM28 (for tripartif motif-containing 28), is a member of the tripartif motif family characterized by three zinc-binding domains, a RING finger, B-boxes and a coiled-coil domain (1,2,4). Like TIF1å, TIF1∫ contains both a Cys/His PHD (plant homeodomain) finger and bromodomain that form a cooperative unit required for transcriptional repression (3,5). TIF1∫ mediates transcriptional control by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors and by binding DNA through its zinc finger (3,6). The human TIF1∫ gene maps to human chromosome 19q13.4 and encodes an 835 amino acid nuclear protein (3,4).

TIF1β Antibody (H-300) Product Citations
See how others have used TIF1β Antibody (H-300) and or TIF1β Antibody (H-300) conjugates.


3 total citations
Loading citations.
 
TIF1β Antibody (H-300) Data
Click on image to enlarge
sc-33186
TIF1β (H-300): sc-33186. Western blot analysis of TIF1β expression in CCRF-CEM nuclear extract.
sc-33186
TIF1β (H-300): sc-33186. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
sc-33186
TIF1β (H-300): sc-33186. Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.
sc-33186
TIF1β (H-300): sc-33186. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear staining of trophoblastic cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
sc-33186
TIF1β (H-300): sc-33186. Western blot analysis of TIF1β expression in non-transfected 293T: sc-117752 (A), human TIF1β transfected 293T: sc-116358 (B) and CCRF-CEM (C) whole cell lysates.
sc-33186
TIF1β (H-300): sc-33186. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of Islets of Langerhans and glandular cells.
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