epitope corresponding to phosphorylated Tyr 793 of NSP3 of human origin
recommended for detection of Tyr 793 phosphorylated NSP3 of human origin, Tyr 787 phosphorylated NSP3 of mouse origin, and correspondingly phosphorylated NSP3 of rat, equine, canine, bovine, porcine and avian origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
NSP3 Background Information The Eph family of receptor tyrosine kinases has been implicated in many developmental patterning processes, including cell segregation, cell migration, and axon guidance. An intermediate that is involved in the signaling pathways of the Eph receptors is novel SH2-containing protein 3 (NSP3, also designated SH2 domain-containing Eph receptor-binding protein 1, SHEP1, Cas, or HEF1 Associated Signal Transducer), expressed in both the embryonic and adult brain. NSP3 contains a Src homology 2 domain that binds to a conserved tyrosine-phosphorylated motif in the juxtamembrane region of the EphB2 receptor. NSP3 may itself be a target of EphB2 kinase activity since it becomes heavily tyrosine-phosphorylated in cells expressing activated EphB2. NSP3 directly links activated, tyrosine-phosphorylated, Eph receptors to small Ras superfamily GTPases.
