CBP Tag Background Information
Plasmid vectors are commonly used for the expression of coding regions of eukaryotic genes in E. coli; such expression vectors often encode hybrid fusion proteins containing a eukaryotic specified protein fused on a tag protein. Antibodies specific for such fusion protein tags are useful for monitoring protein expression both in plaques and on Western immunoblots, as well as for immunoaffinity purification of proteins expressed in E. coli. Antibodies are offered to a range of fusion protein tags, including GST, His, Omni, b-Gal, S-probe, CBP, MBP-probe, Thio-probe, Luciferase, Green Fluorescent Protein (GFP), Yellow Fluorescent Protein (YFP) and the novel CruzTag series of fusion protein tags. Fields and coworkers have developed a unique “two-hybrid” system using GAL4 fusions in yeast to identify specific protein-protein interactions. The more recently developed Lex A system is a modificaiton of the original Fields system. Antibodies are offered to several fusion protein tags that are used in yeast “two-hybrid” vectors, including GAL4-TA, GAL4(DBD), Lex A, B42, CBP and VP16.
