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home » Synthesis and Degradation » PFK Antibody / PFK Antibodies » p-PFK-2 car (Ser 483): sc-32967

p-PFK-2 car (Ser 483) Antibody: sc-32967

  |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml
  • epitope corresponding to phosphorylated Ser 483 of PFK-2 car of human origin
  • recommended for detection of Ser 483 phosphorylated cardiac-type PFK-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine and bovine
  • blocking peptide, sc-32967 P
 
Additional PFK Antibodies ...
 
Ordering Information
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human PFKFB2 5208 1q32.1 NM_001018053, NM_006212 O60825
 171835
Mouse Pfkfb2 18640 1 E4 P70265
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-PFK-2 car (Ser 483) sc-32967 200 µg/ml $279
p-PFK-2 car (Ser 483) P sc-32967 P
(peptide)
 100 µg/0.5 ml $61
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
PFK-2 car siRNA (h) sc-44675 10 µM $258
PFK-2 car siRNA (m) sc-44676 10 µM $258
PFK-2 car (h)-PR sc-44675-PR 10 µM $23
PFK-2 car (m)-PR sc-44676-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
PFK-2 car shRNA Plasmid (h) sc-44675-SH 20 µg $520
PFK-2 car shRNA Plasmid (m) sc-44676-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
PFK-2 car shRNA (h) Lentiviral Particles sc-44675-V 200 µl $625
PFK-2 car shRNA (m) Lentiviral Particles sc-44676-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
PFK-2 car (m): 293T Lysate sc-122507 100µg/200µl $205
PFK-2 car (m2): 293T Lysate sc-122508 100µg/200µl $205

PFK-2 car Background Information
Phosphofructose kinase-2 (PFK-2) belongs to the phosphoglycerate mutase family and is required for the activation of cellular glycolysis. Within the glycolysis pathway, PFK-2 regulates the synthesis and degradation of fructose 2,6-bisphosphate (F2,6BP) by enzymatically catalyzing the phosphorylation of fructose-6-phosphate to form F2,6BP. F2,6BP functions as a potent activator of 6-phosphofructo-1-kinase that can then activate the glycolysis pathway. Various tissue-specific isoforms of PFK-2 are expressed and they are differentially regulated and function as homodimers. A unique isoform, iPFK-2, is induced following proinflammatory stimuli, and it is also constituitively expressed in a variety of carcinoma cell lines, where it leads to an accumulation of intracellular F2,6BP. Activation of PFK-2 cardiac (PFK-2 car) by insulin results from Ser 466 and Ser 483 phosphorylation and requires a PDK-1-activated protein kinase other than PKB.
p-PFK-2 car (Ser 483)
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sc-32967
p-PFK-2 car (Ser 483): sc-32967. Western blot analysis of p-PFK-2 car phosphorylation in non-transfected: sc-117752 (A) and mouse p-PFK-2 car transfected: sc-122507 (B) 293T whole cell lysates.
sc-32967
p-PFK-2 car (Ser 483): sc-32967. Western blot analysis of p-PFK-2 car phosphorylation in non-transfected: sc-117752 (A) and mouse p-PFK-2 car transfected: sc-122508 (B) 293T whole cell lysates.
sc-32967
Western blot analysis of PFK-2 car phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse PFK-2 car transfected: sc-122507 (B,E) and lambda protein phosphatase (sc-200312A) treated mouse PFK-2 car transfected: sc-122507 (C,F) 293T whole cell lysates. Antibodies tested include p-PFK-2 car (Ser 483): sc-32967 (A,B,C) and PFK-2 car (Y-13): sc-50956 (D,E,F).
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