epitope corresponding to phosphorylated Thr 1462 of tuberin of human origin
recommended for detection of Thr 1462 phosphorylated tuberin of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine and porcine
tuberin Background Information Hamartin (TSC1) and tuberin (TSC2, LAM, TSC4) can form a functional complex and negatively regulate cell growth by inhibiting protein synthesis. Tuberous sclerosis complex (TSC) is a genetic disorder that arises through mutations in the hamartin and tuberin genes. The phosphoinositide 3-kinase (PI3K)/Akt pathway phosphorylates tuberin on serine-939 and threonine-1462 and inhibits the tumor suppressor function of the TSC complex. Anisomycin stimulates phosphorylation of serine-1210 of tuberin via the p38-MK2 kinase cascade. Phosphorylation of tuberin by MK2 creates a 14-3-3 binding site. Phosphorylation of hamartin regulates the function of the hamartin-tuberin complex during the G2/M phase of the cell cycle.
