GM-CSF Background Information Colony stimulating factors (CSFs) were initially characterized by their ability to stimulate in vitro colony formation by hematopoietic progenitor cells in semisolid media. Several of these CSFs have been assigned an interleukin number, while three (GM-CSF, G-CSF and M-CSF) have retained their CSF designations. The human granulocyte-macrophage colony stimulating factor (GM-CSF) is a pleiotropic cytokine with a 17 amino acid signal peptide that is cleaved to produce the mature form of 127 amino acids. The mature murine GM-CSF protein is 124 amino acids and shares 60% homology with the human GM-CSF protein. GM-CSF is a glycoprotein that can stimulate the proliferation of hematopoietic cells including granulocytes and macrophages. It has been shown to promote the phosphorylation of cPLA2 in human neutrophils. The phosphorylation of cPLA2 may be accompanied by an increase in enzyme activity.
GM-CSF (1D11)
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Western blot analysis of human recombinant GM-CSF immunoprecipitated with GM-CSF (1D11): sc-32805 and detected with GM-CSF (B6-2-hGMCSF): sc-32753. Note presence of heavy and light chains of IgG.
