MyoD Antibody (5.8A): sc-32758

MyoD Antibody (5.8A) is a mouse monoclonal IgG1 kappa light chain antibody specifically engineered to recognize Myogenic Differentiation 1 (MyoD), a pivotal transcription factor in the Myogenic Regulatory Factors (MRFs) family, which also includes myogenin, Myf5, and MRF4 (herculin). MyoD monoclonal antibody (5.8A) is raised against recombinant mouse MyoD, targeting an epitope located between amino acids 180-189, ensuring precise binding to the MyoD protein. MyoD plays an essential role in muscle cell differentiation by binding to E-box sequences (CANNTG) in the DNA, thereby regulating the expression of genes critical for muscle development and regeneration. By forming heterodimers with E proteins such as E2A, IF2, and HEB, MyoD activates the transcription of muscle-specific genes, making MyoD a key regulator in myogenesis. MyoD monoclonal antibody (5.8A) is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP) applications across multiple species, including mouse, rat, and human, without cross-reacting with other members of the MyoD family. Available in both conjugated and non-conjugated forms, anti-MyoD antibody (5.8A) is an indispensable tool for scientists studying the molecular mechanisms underlying muscle differentiation and regeneration.

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MyoD Antibody (5.8A) References:

1. FHL3 binds MyoD and negatively regulates myotube formation.  |  Cottle, DL., et al. 2007. J Cell Sci. 120: 1423-35. PMID: 17389685
2. Herculin, a fourth member of the MyoD family of myogenic regulatory genes.  |  Miner, JH. and Wold, B. 1990. Proc Natl Acad Sci U S A. 87: 1089-93. PMID: 2300571
3. Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12.  |  Braun, T., et al. 1990. EMBO J. 9: 821-31. PMID: 2311584
4. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.  |  Wright, WE., et al. 1989. Cell. 56: 607-17. PMID: 2537150
5. Identification of MRF4: a new member of the muscle regulatory factor gene family.  |  Rhodes, SJ. and Konieczny, SF. 1989. Genes Dev. 3: 2050-61. PMID: 2560751
6. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.  |  Braun, T., et al. 1989. EMBO J. 8: 701-9. PMID: 2721498
7. Finding MyoD and lessons learned along the way.  |  Lassar, AB. 2017. Semin Cell Dev Biol. 72: 3-9. PMID: 29097153
8. Master control: transcriptional regulation of mammalian Myod.  |  Wardle, FC. 2019. J Muscle Res Cell Motil. 40: 211-226. PMID: 31301002
9. MyoD-Induced Trans-Differentiation: A Paradigm for Dissecting the Molecular Mechanisms of Cell Commitment, Differentiation and Reprogramming.  |  Battistelli, C., et al. 2022. Cells. 11: PMID: 36359831
10. HLH forced dimers: tethering MyoD to E47 generates a dominant positive myogenic factor insulated from negative regulation by Id.  |  Neuhold, LA. and Wold, B. 1993. Cell. 74: 1033-42. PMID: 7691411
11. A cellular factor stimulates the DNA-binding activity of MyoD and E47.  |  Thayer, MJ. and Weintraub, H. 1993. Proc Natl Acad Sci U S A. 90: 6483-7. PMID: 8393567
12. Use of a conditional MyoD transcription factor in studies of MyoD trans-activation and muscle determination.  |  Hollenberg, SM., et al. 1993. Proc Natl Acad Sci U S A. 90: 8028-32. PMID: 8396258

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MyoD Antibody (5.8A) has 140 citations in a variety of scientific publications.

Primary antibodies like Anti-MyoD Antibody (5.8A) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MyoD Antibody (5.8A) Specifications

Alternate Product Name: MyoD

Product Clone Name: 5.8A

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 180-189

Epitope Target: MyoD

Epitope Species: mouse

Product Reactivity/Detection: MyoD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 140 citations in various scientific publications

Available Conjugates/Forms for MyoD Antibody (5.8A): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, X: TransCruz Reagent