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home » Neurobiology » NF Antibody / NF Antibodies » p-NF-H (RNF405): sc-32730

p-NF-H (RNF405) Antibody: sc-32730

  |  Datasheet
  • mouse monoclonal IgG2a; 200 µg/ml
  • raised against a neurofilament NF-H protein isolated from a cytoskeletal preparation from calf brain
  • recommended for detection of phosphorylated NF-H of mouse, rat, human and Xenopus origin by WB, IP and IF; also reactive with additional species, including bovine
 
Additional NF Antibodies ...
 
Ordering Information
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human NEFH 4744 22q12.2 NM_021076 P12036
 162230
Mouse Nefh 380684 11 A1 P19246
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-NF-H (RNF405) sc-32730 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NF-H siRNA (h) sc-42068 10 µM $258
NF-H siRNA (m) sc-42069 10 µM $258
NF-H (h)-PR sc-42068-PR 10 µM $23
NF-H (m)-PR sc-42069-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NF-H shRNA Plasmid (h) sc-42068-SH 20 µg $520
NF-H shRNA Plasmid (m) sc-42069-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NF-H shRNA (h) Lentiviral Particles sc-42068-V 200 µl $625
NF-H shRNA (m) Lentiviral Particles sc-42069-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
rat brain extract sc-2392 500 µg/200 µl $104
mouse brain extract sc-2253 500 µg/200 µl $104
NF-H (h): 293T Lysate sc-111457 100µg/200µl $205
rat cerebellum extract sc-2398 500 µg/200 µl $104

NF-H Background Information
Neurofilament-H (for neurofilament heavy polypeptide, or NF-H), a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3 and stress-activated protein kinase g. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease.
p-NF-H (RNF405)
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sc-32730
p-NF-H (RNF405): sc-32730. Western blot analysis of NF-H phosphorylation in rat brain (A), mouse brain (B), rat cerebellum (C) and mouse cerebellum (D) tissue extracts.
sc-32730
p-NF-H (RNF405): sc-32730. Western blot analysis of NF-H prosphorylation in non-transfected: sc-117752 (A) and human NF-H transfected: sc-111457 (B) 293T whole cell lysates.
sc-32730
Western blot analysis of NF-H phosphorylation in non-transfected: sc-117752 (A,D), untreated human NF-H transfected: sc-111457 (B,E) and lambda protein phosphatase (sc-200312A) treated human NF-H transfected: sc-111457 (C,F) 293T whole cell lysates. Antibodies tested include p-NF-H (RNF405): sc-32730 (A,B,C) and NF-H (2D2): sc-20014 (D,E,F).
sc-32730
Western blot analysis of NF-H phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) rat brain tissue extracts. Antibodies tested include p-NF-H (RNF405): sc-32730 (A,B) and NF-H (2D2): sc-20014 (C,D).
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