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2,2,7-trimethylguanosine (K121) Antibody: sc-32724 |
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- mouse monoclonal IgG1; 200 µg/ml
- raised against trimethylguanosine-keyhole limpet hemocyanin conjugates
- recommended for detection of 5` terminal 2,2,7-trimethylguanosine of all major snRNAs of mouse, rat and human origin by IP and IF
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2,2,7-trimethylguanosine Background Information
Pre-mRNA splicing is a critical step in the post-transcriptional regulation of gene expression. Several protein complexes are involved in proper mRNA splicing and transport. The small nuclear ribonucleoprotein particles (snRNPs) interact with the SRm160/300 splicing coactivator complex to form a large RNA spliceosome. 2,2,7-trimethylguanosine is a nucleoside at the 5’ terminal cap site of the snRNA (small ribonucleoprotein RNA) componenet of U1, U2, U4/U6 and U5 nuclear snRNPs. U-type snRNPs mediate intron splicing of precursor mRNA. Antibodies recognizing the 5’ terminal cap motif can inhibit mRNA splicing. Anti-trimethylguanosine (TMG) monoclonal, K121, can immunoprecipitate all major nuclear snRNPs.
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2,2,7-trimethylguanosine (K121)
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2,2,7-trimethylguanosine (K121): sc-32724. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
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