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p-RKIP (rSer 153) Antibody: sc-32622 |
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- rabbit polyclonal IgG, 200µg/ml
- epitope corresponding to phosphorylated Ser 153 of RKIP of rat origin
- recommended for detection of Ser 153 phosphorylated RKIP of rat origin by WB, IP, IF and ELISA
- blocking peptide, sc-32622 P
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p-RKIP Background Information
Raf kinase inhibitory protein (RKIP, PEBP) is a modulator of the Raf/MAPK signaling cascade and a suppressor of metastatic cancer. RKIP inhibits MAPK by preventing association of Raf-1 and p21-activated kinase (PAK), and blocking phosphorylation of the Raf-1 kinase domain by PAK and Src kinases. After G protein receptor stimulation, RKIP can dissociate Raf-1 and associate with GRK2, thereby blocking GRK2 activity. This switch is triggered by protein kinase C (PKC)-dependent phosphorylation of the RKIP on Serine 153. RKIP Serine 153 phosphorylation by PKC in response to phorbol ester or epidermal growth factor causes release of RKIP from Raf-1. RKIP antagonizes the signal transduction pathways that mediate the activation of NFkB in response to stimulation with TNF-a or interleukin-1 b. |
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p-RKIP (rSer 153)
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Western blot analysis of RKIP phosphorylation in untreated (A,C) and lambda protein phosphatase ( sc-200312A) treated (B,D) rat liver tissue extracts. Antibodies tested include p-RKIP (rSer 153): sc-32622 (A,B) and RKIP (FL-187): sc-28837 (C,D). Western blot analysis of RKIP phosphorylation in untreated (A,C) and lambda protein phosphatase ( sc-200312A) treated (B,D) rat adrenal tissue extracts. Antibodies tested include p-RKIP (rSer 153): sc-32622 (A,B) and RKIP (FL-187): sc-28837 (C,D). Western blot analysis of RKIP phosphorylation in untreated (A,C) and lambda protein phosphatase ( sc-200312A) treated (B,D) PC-12 whole cell lysates. Antibodies tested include p-RKIP (rSer 153): sc-32622 (A,B) and RKIP (FL-187): sc-28837 (C,D). |
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