epitope mapping within an internal region of TEF-4 of human origin
recommended for detection of TEF-4 of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine and bovine
blocking peptide, sc-32428 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-32428 X, 200 µg/0.1 ml
TEF-4 Background Information The transcriptional enhancer factor (TEF)/TEAD family includes TEF-1, TEF-3, TEF-4 and TEF-5. These proteins share a highly conserved 68-amino acid TEA/ATTS DNA-binding domain, which binds to SV40 GT-IIC (GGAATG), SphI (AGTATG), SphII (AGCATG), and muscle-specific M-CAT (GGTATG) enhansons. TEFs are differentially expressed in human cultured cell lines and mouse embryonic and extra-embryonic tissues. Specifically, TEF-4 is strongly coexpressed with TEF-1 in mouse mitotic neuroblasts and is also detected in the gut and the nephrogenic region of the kidney. TEF-4 associates with the powerful transcriptional coactivator YAP65 to mediate mitogenic signals. In addition, TEF-4 promotes the activation of the CTP:phosphocholine cytidylyltransferase (CCT) alpha protein, which is the rate-limiting enzyme of phosphatidylcholine biosynthesis, by enhancing the transcriptional activity of Ets-1.