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- goat polyclonal IgG, 200 µg/ml
- epitope mapping near the N-terminus of ALAS-E of human origin
- recommended for detection of precursor and mature ALAS-E of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
- blocking peptide, sc-32334 P
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Ordering Information
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ALAS-E Background Information
5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia. |
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ALAS-E (N-20)
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ALAS-E (N-20): sc-32334. Western blot analysis of ALAS-E expression in non-transfected: sc-117752 (A) and human ALAS-E transfected: sc-114245 (B) 293T whole cell lysates. |
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