Cytokeratin 5/8 (RCK102) Antibody sc-32328

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Cytokeratin 5/8 (RCK102) Antibody: sc-32328	\| Datasheet

mouse monoclonal IgG₁; 200 µg/ml
raised against the human lung cancer cell line, MR21
recommended for detection of Cytokeratin 5 and cytokeratin 8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; may cross-react with Cytokeratin 18
fluorescein (sc-32328 FITC) and phycoerythrin (sc-32328 PE) conjugates for FCM, 100 tests

Additional Cytokeratin Antibodies ...

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WB IP IF IHC(P) FCM

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

Human	KRT18	3875	12q13.13	NM_000224, NM_199187	P05783	215600
Mouse	Krt8	16691	15 F3		P11679	N/A
Mouse	Krt5	110308	15 F2		Q922U2	N/A

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Ordering Information

Product Name	Catalog #	Unit	Price
Cytokeratin 5/8 (RCK102)	sc-32328	200 µg/ml	$279
Cytokeratin 5/8 (RCK102) FITC	sc-32328 FITC	100 tests in 2 ml	$292
Cytokeratin 5/8 (RCK102) PE	sc-32328 PE	100 tests in 2ml	$303

WB Positive Control Cell Lysates (click product name for more information)

Product Name	Catalog #	Unit	Price
HeLa Whole Cell Lysate	sc-2200	500 µg/200 µl	$104
SK-BR-3 Cell Lysate	sc-2218	500 µg/200 µl	$104
A-431 Whole Cell Lysate	sc-2201	500 µg/200 µl	$104
Cytokeratin 18 (h3): 293 Lysate	sc-112918	100µg/200µl	$205
MCF7 Whole Cell Lysate	sc-2206	500 µg/200 µl	$104
Cytokeratin 18 (h): 293 Lysate	sc-110494	100µg/200µl	$205
T24 Cell Lysate	sc-2292	500 µg/200 µl	$104
Hep G2 Cell Lysate	sc-2227	500 µg/200 µl	$104

Cytokeratin 5/8 Background Information
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin 5 is expressed in normal basal cells. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB). Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors Cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas.

Cytokeratin 5/8 (RCK102) Product Citations

See how others have used Cytokeratin 5/8 (RCK102): sc-32328 antibody and or Cytokeratin 5/8 (RCK102) antibody conjugates.

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Cytokeratin 5/8 (RCK102)

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Cytokeratin 5/8 (RCK102): sc-32328. Western blot analysis of Cytokeratin 5/8 expression in SK-BR-3 (A) and A-431 (B) whole cell lysates.

Cytokeratin 5/8 (RCK102): sc-32328. Immunoperoxidase staining of human squamous cell carcinoma of the lung. Kindly provided by MUBio, Netherlands.

Cytokeratin 5/8 (RCK102): sc-32328. Western blot analysis of Cytokeratin 5 and Cytokeratin 8 expression in SK-BR-3 (A), HeLa (B) and A-431 (C) whole cell lysates.

Cytokeratin 5/8 (RCK102) PE: sc-32328 PE. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG₁: sc-2866.

Cytokeratin 5/8 (RCK102): sc-32328. Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tumor showing cytoskeletal localization.

Cytokeratin 5/8 (RCK102): sc-32328. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.

Cytokeratin 5/8 (RCK102): sc-32328. Western blot analysis of Cytokeratin 5/8 expression in Hep G2 whole cell lysate.

Cytokeratin 5/8 (RCK102): sc-32328. Western blot analysis of Cytokeratin 18 expression in non-transfected: sc-117752 (A) and human Cytokeratin 18 transfected: sc-112918 (B) 293T whole cell lysates.

Cytokeratin 5/8 (RCK102): sc-32328. Western blot analysis of Cytokeratin 5/8 expression in T24 whole cell lysate.

Cytokeratin 5/8 (RCK102): sc-32328. Western blot analysis of Cytokeratin 18 expression in non-transfected 293: sc-110760 (A), human Cytokeratin 18 transfected 293: sc-110494 (B) and HeLa (C) whole cell lysates.

Cytokeratin 5/8 (RCK102): sc-32328. Western blot analysis of Cytokeratin 5/8 expression in SK-BR-3 whole cell lysate.

Cytokeratin 5/8 (RCK102): sc-32328. Western blot analysis of Cytokeratin 5/8 expression in MCF7 whole cell lysate.

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Cytokeratin 5/8 (RCK102) Antibody: sc-32328