Vimentin Antibody (RV202): sc-32322

Vimentin Antibody (RV202) is a mouse monoclonal IgG1 kappa light chain antibody raised against cytoskeletal vimentin extracts from calf lens tissue, a critical intermediate filament protein essential for cellular structure and function. Vimentin mouse monoclonal antibody (RV202) recognizes an epitope within the vimentin immunogen sequence and demonstrates broad species cross-reactivity, including mouse, rat, and human samples, in Western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry on paraffin-embedded sections, and flow cytometry applications. Anti-vimentin monoclonal antibody (RV202) is available in non-conjugated form and multiple conjugated forms including horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, agarose, and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. Vimentin maintains cell shape, stabilizes organelles, and supports cellular motility through a complex network of protein interactions. Among these interactions, vimentin forms important associations with integrin β1, which facilitates cell adhesion and migration, processes crucial during wound healing and cancer metastasis. Vimentin also binds plectin, a crosslinking protein that anchors vimentin to other cytoskeletal components such as actin and microtubules, thereby enhancing cellular and tissue structural integrity. The interaction between vimentin and 14-3-3 proteins influences various signaling pathways related to cell survival and apoptosis. These molecular partnerships highlight vimentin′s significance in cytoskeletal organization and epithelial-to-mesenchymal transition (EMT), a process fundamental to cancer progression and metastasis. Vimentin (RV202) antibody serves as a valuable tool for investigating these complex cellular processes in cytoskeletal dynamics, cancer biology, and cellular diagnostics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Vimentin Antibody (RV202) References:

1. Vimentin fibers orient traction stress.  |  Costigliola, N., et al. 2017. Proc Natl Acad Sci U S A. 114: 5195-5200. PMID: 28465431
2. Vimentin as antigenic target in autoimmunity: A comprehensive review.  |  Musaelyan, A., et al. 2018. Autoimmun Rev. 17: 926-934. PMID: 30009963
3. Vimentin regulates the assembly and function of matrix adhesions.  |  Ostrowska-Podhorodecka, Z. and McCulloch, CA. 2021. Wound Repair Regen. 29: 602-612. PMID: 33887795
4. RAB7A Regulates Vimentin Phosphorylation through AKT and PAK.  |  Romano, R., et al. 2021. Cancers (Basel). 13: PMID: 34066419
5. Biomarker Potential of Vimentin in Oral Cancers.  |  Mogre, S., et al. 2022. Life (Basel). 12: PMID: 35207438
6. Impact of Vimentin on Regulation of Cell Signaling and Matrix Remodeling.  |  Ostrowska-Podhorodecka, Z., et al. 2022. Front Cell Dev Biol. 10: 869069. PMID: 35359446
7. Pathophysiological Role of Vimentin Intermediate Filaments in Lung Diseases.  |  Surolia, R. and Antony, VB. 2022. Front Cell Dev Biol. 10: 872759. PMID: 35573702
8. Extracellular vimentin mimics VEGF and is a target for anti-angiogenic immunotherapy.  |  van Beijnum, JR., et al. 2022. Nat Commun. 13: 2842. PMID: 35606362
9. Targeting the KRT16-vimentin axis for metastasis in lung cancer.  |  Wang, W., et al. 2023. Pharmacol Res. 193: 106818. PMID: 37315823
10. RNF26 binds perinuclear vimentin filaments to integrate ER and endolysosomal responses to proteotoxic stress.  |  Cremer, T., et al. 2023. EMBO J. 42: e111252. PMID: 37519262

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Vimentin Antibody (RV202) has 199 citations in a variety of scientific publications.

Primary antibodies like Anti-Vimentin Antibody (RV202) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Vimentin Antibody (RV202) Specifications

Alternate Product Name: Vimentin

Product Clone Name: RV202

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Vimentin

Epitope Species: bovine

Product Reactivity/Detection: Vimentin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 199 citations in various scientific publications

Available Conjugates/Forms for Vimentin Antibody (RV202): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, PerCP: Peridinin Chlorophyll Protein Complex with Cyanin-5.5

The preferred Vimentin antibody is Vimentin Antibody (V9): sc-6260.