Y14 Antibody (4C4): sc-32312

Y14 Antibody (4C4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Y14 protein of human and Xenopus laevis origin, as well as RBM8 protein of mouse and rat origin, by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Y14 (4C4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Y14 protein plays a crucial role in the exon junction complex (EJC), a multiprotein assembly that forms approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. EJC is essential for mRNA export, cytoplasmic localization, and the regulation of nonsense-mediated mRNA decay, ensuring that only properly processed mRNAs are translated. Y14, along with Aly/REF and Magoh, constitutes the core of EJC, remaining bound to spliced mRNAs in the cytoplasm until translation occurs. This interaction communicates the processing history of mRNA, including the positions of removed introns, to the cytoplasm. The gene encoding human Y14 produces three transcripts, and Y14 protein is ubiquitously expressed, predominantly localized in the nucleus, where Y14 co-localizes with oskar mRNA at the posterior pole of the cell, highlighting its importance in mRNA regulation and cellular function.

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Y14 Antibody (4C4) References:

1. MAGOH interacts with a novel RNA-binding protein.  |  Zhao, XF., et al. 2000. Genomics. 63: 145-8. PMID: 10662555
2. Cloning and gene expression of a novel human ribonucleoprotein.  |  Conklin, DC., et al. 2000. Biochim Biophys Acta. 1492: 465-9. PMID: 11004516
3. Nuclear mRNA binding proteins couple pre-mRNA splicing and post-splicing events.  |  Kim, VN. and Dreyfuss, G. 2001. Mol Cells. 12: 1-10. PMID: 11561715
4. Drosophila Y14 shuttles to the posterior of the oocyte and is required for oskar mRNA transport.  |  Hachet, O. and Ephrussi, A. 2001. Curr Biol. 11: 1666-74. PMID: 11696323
5. Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex.  |  Kataoka, N., et al. 2001. EMBO J. 20: 6424-33. PMID: 11707413
6. The protein Mago provides a link between splicing and mRNA localization.  |  Le Hir, H., et al. 2001. EMBO Rep. 2: 1119-24. PMID: 11743026
7. Translation is required to remove Y14 from mRNAs in the cytoplasm.  |  Dostie, J. and Dreyfuss, G. 2002. Curr Biol. 12: 1060-7. PMID: 12121612
8. 5' exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly.  |  Reichert, VL., et al. 2002. Genes Dev. 16: 2778-91. PMID: 12414731
9. Function and pathological implications of exon junction complex factor Y14.  |  Chuang, TW., et al. 2015. Biomolecules. 5: 343-55. PMID: 25866920
10. Y14 governs p53 expression and modulates DNA damage sensitivity.  |  Lu, CC., et al. 2017. Sci Rep. 7: 45558. PMID: 28361991
11. The Y14-p53 regulatory circuit in megakaryocyte differentiation and thrombocytopenia.  |  Su, CH., et al. 2021. iScience. 24: 103368. PMID: 34816104
12. Co-phase separation of Y14 and RNA in vitro and its implication for DNA repair.  |  Yu, CL., et al. 2023. RNA. 29: 1007-1019. PMID: 37001915

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Y14 Antibody (4C4) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-Y14 Antibody (4C4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Y14 Antibody (4C4) Specifications

Alternate Product Name: Y14

Product Clone Name: 4C4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: Y14

Epitope Species: human

Product Reactivity/Detection: Y14 of human and Xenopus laevis origin, and RBM8 of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for Y14 Antibody (4C4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin