α-Actin Antibody (1A4): sc-32251
- α-Actin Antibody (1A4) is a mouse monoclonal IgG2a κ α-Actin antibody, cited in 414 publications, provided at 200 µg/ml
- raised against an N-terminal decapeptide of α-Actin of human origin
- alpha-Actin Antibody (1A4) is recommended for detection of smooth muscle α-Actin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)
- Anti-alpha-Actin Antibody (1A4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for α-Actin Antibody (1A4) for WB and IHC(P) applications. These reagents are now offered in bundles with α-Actin Antibody (1A4) (see ordering information below).
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α-Actin Antibody (1A4) is a mouse monoclonal IgG2a kappa light chain antibody that detects alpha-Actin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-alpha-Actin antibody (1A4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Actin plays a vital role in cellular structure and function as a key component of the cytoskeleton, providing mechanical support and shape to cells. Alpha-Actin forms dynamic filaments in the cytoplasm that are essential for various cellular processes, including motility, division, and intracellular transport. Rapid polymerization and depolymerization of alpha-Actin allows cells to adapt shape and movement in response to environmental cues, making this protein crucial for processes such as wound healing and immune responses. Alpha-Actin contributes significantly to the contractile apparatus in muscle cells, enabling muscle contraction and relaxation. Members of the small GTPase family tightly control alpha-Actin dynamics by orchestrating the assembly and disassembly of actin filaments, ensuring proper cellular function and response to stimuli.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
α-Actin Antibody (1A4) References:
- Muscle disease caused by mutations in the skeletal muscle alpha-actin gene (ACTA1). | Sparrow, JC., et al. 2003. Neuromuscul Disord. 13: 519-31. PMID: 12921789
- Cardiac α-actin over-expression therapy in dominant ACTA1 disease. | Ravenscroft, G., et al. 2013. Hum Mol Genet. 22: 3987-97. PMID: 23736297
- Nuclear Smooth Muscle α-actin in Vascular Smooth Muscle Cell Differentiation. | Kwartler, CS., et al. 2023. Res Sq.. PMID: 36909460
- Smooth muscle α-actin missense variant promotes atherosclerosis through modulation of intracellular cholesterol in smooth muscle cells. | Kaw, K., et al. 2023. Eur Heart J. 44: 2713-2726. PMID: 37377039
- Nuclear Smooth Muscle α-actin Participates in Vascular Smooth Muscle Cell Differentiation. | Kwartler, CS., et al. 2023. Nat Cardiovasc Res. 2: 937-955. PMID: 38919852
- Role of microtubule-associated proteins in the control of microtubule assembly. | Maccioni, RB. and Cambiazo, V. 1995. Physiol Rev. 75: 835-64. PMID: 7480164
- Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. | Nobes, CD. and Hall, A. 1995. Cell. 81: 53-62. PMID: 7536630
- A discourse on modeling F-actin. | Schutt, CE., et al. 1995. J Struct Biol. 115: 186-98. PMID: 7577239
- Actin cytoskeleton. Setting the pace of cell movement. | Barkalow, K. and Hartwig, JH. 1995. Curr Biol. 5: 1000-2. PMID: 8542272
- The origins and evolution of eukaryotic proteins. | Doolittle, RF. 1995. Philos Trans R Soc Lond B Biol Sci. 349: 235-40. PMID: 8577833
- Elastic fibres are an essential component of human placental stem villous stroma and an integrated part of the perivascular contractile sheath. | Graf, R., et al. 1996. Cell Tissue Res. 283: 133-41. PMID: 8581953
- Actin bundles in human hair follicles as revealed by confocal laser microscopy. | Furumura, M. and Ishikawa, H. 1996. Cell Tissue Res. 283: 425-34. PMID: 8593672
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α-Actin Antibody (1A4) | sc-32251 | 200 µg/ml | $322.00 | |||
α-Actin Antibody (1A4): m-IgG Fc BP-HRP Bundle | sc-528446 | 200 µg Ab; 10 µg BP | $361.00 | |||
α-Actin Antibody (1A4): m-IgGκ BP-HRP Bundle | sc-520826 | 200 µg Ab, 40 µg BP | $361.00 | |||
α-Actin Antibody (1A4): m-IgG2a BP-HRP Bundle | sc-547102 | 200 µg Ab; 10 µg BP | $361.00 | |||
α-Actin Antibody (1A4) AC | sc-32251 AC | 500 µg/ml, 25% agarose | $424.00 | |||
α-Actin Antibody (1A4) HRP | sc-32251 HRP | 200 µg/ml | $322.00 | |||
α-Actin Antibody (1A4) FITC | sc-32251 FITC | 200 µg/ml | $336.00 | |||
α-Actin Antibody (1A4) PE | sc-32251 PE | 200 µg/ml | $349.00 | |||
α-Actin Antibody (1A4) Alexa Fluor® 488 | sc-32251 AF488 | 200 µg/ml | $364.00 | |||
α-Actin Antibody (1A4) Alexa Fluor® 546 | sc-32251 AF546 | 200 µg/ml | $364.00 | |||
α-Actin Antibody (1A4) Alexa Fluor® 594 | sc-32251 AF594 | 200 µg/ml | $364.00 | |||
α-Actin Antibody (1A4) Alexa Fluor® 647 | sc-32251 AF647 | 200 µg/ml | $364.00 | |||
α-Actin Antibody (1A4) Alexa Fluor® 680 | sc-32251 AF680 | 200 µg/ml | $364.00 | |||
α-Actin Antibody (1A4) Alexa Fluor® 790 | sc-32251 AF790 | 200 µg/ml | $364.00 |