Welcome to Santa Cruz Biotechnology!

α-Actin Antibody (1A4): sc-32251


  See 9 reviews  |  Add your review

  • α-Actin Antibody (1A4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
  • raised against an N-terminal decapeptide of smooth muscle α-Actin of human origin
  • recommended for detection of smooth muscle α-Actin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with Actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial) and striated muscle (α-sarcomeric)
  • agarose (sc-32251 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-32251 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-32251 PE), fluorescein (sc-32251 FITC), Alexa Fluor® 488 (sc-32251 AF488) or Alexa Fluor® 647 (sc-32251 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, m-IgGκ BP-FITC: sc-516140, m-IgGκ BP-PE: sc-516141, or m-IgGκ BP-B: sc-516142, our highly recommended recombinant alternatives to conventional secondary anti-mouse IgG reagents.
  • See 94 citations for α-Actin Antibody (1A4)

See additional Actin Antibodies including Actin, α-Actin, β-Actin, γ-Actin, κ-Actin, Muscle Actin and Smooth Muscle Actin.

Also see chemical Actin Inhibitors for functional analysis of cellular responses to Actin.

Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
α-Actin Antibody (1A4) sc-32251 200 µg/ml $279
α-Actin Antibody (1A4) AC sc-32251 AC 500 µg/ml, 25% ag $366
α-Actin Antibody (1A4) Alexa Fluor® 488 sc-32251 AF488 200 µg/ml $314
α-Actin Antibody (1A4) Alexa Fluor® 647 sc-32251 AF647 200 µg/ml $314
α-Actin Antibody (1A4) FITC sc-32251 FITC 200 µg/ml $292
α-Actin Antibody (1A4) HRP sc-32251 HRP 200 µg/ml $279
α-Actin Antibody (1A4) PE sc-32251 PE 200 µg/ml $303
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ACTA2 siRNA (h) sc-43590 10 µM $258
ACTA2 siRNA (m) sc-43591 10 µM $258
ACTA2 (h)-PR sc-43590-PR 10 µM, 20 µl $23
ACTA2 (m)-PR sc-43591-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ACTA2 shRNA Plasmid (h) sc-43590-SH 20 µg $520
ACTA2 shRNA Plasmid (m) sc-43591-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ACTA2 shRNA (h) Lentiviral Particles sc-43590-V 200 µl $625
ACTA2 shRNA (m) Lentiviral Particles sc-43591-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
A-10 Cell Lysate sc-3806 500 µg/200 µl $104
IMR-32 Cell Lysate sc-2409 500 µg/200 µl $104
mouse heart extract sc-2254 500 µg/200 µl $104
rat brain extract sc-2392 500 µg/200 µl $104
BC3H1 Cell Lysate sc-2299 500 µg/200 µl $104
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
Sol8 Cell Lysate sc-2249 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ACTA2 59 10q23.31 NM_001141945, NM_001613 P62736
Mouse Acta2 11475 19 C1 NM_007392 P62737

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β- and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

α-Actin Antibody (1A4) Product Reviews

9 total reviews.
Add your review!
Loading Reviews.

α-Actin Antibody (1A4) Product Citations
See how others have used α-Actin Antibody (1A4) and or α-Actin Antibody (1A4) conjugates.

94 total citations
Loading citations.
α-Actin Antibody (1A4) Data
Click on image to enlarge
α-Actin (1A4) HRP: sc-32251 HRP. Direct western blot analysis of α-Actin expression in C2C12 (A), A-10 (B), NIH/3T3 (C), BC3H1 (D) and Sol8 (E) whole cell lysates and human heart tissue extract (F).
α-Actin (1A4): sc-32251. Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue showing cytoplasmic and cytoskeletal staining of smooth muscle cells.
α-Actin (1A4): sc-32251. Western blot analysis of α-Actin expression in IMR-32 (A) and C2C12 (B) whole cell lysates.
α-Actin (1A4): sc-32251. Western blot analysis of α-Actin expression in A-10 (A) and NIH/3T3 (B) whole cell lysates.