MHC class I Antibody (W6/32): sc-32235
- MHC class I Antibody (W6/32) is a mouse monoclonal IgG2a κ MHC class I antibody, cited in 39 publications, provided at 200 µg/ml
- MHC class I Antibody (W6/32) is recommended for detection of HLA-A, HLA-B and HLA-C of human origin by WB, IP, IF and FCM
- recognizing the W6/32 antigenic determinant common to HLA-A, B and C antigens of human origin
- Anti-MHC class I Antibody (W6/32) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to biotin for WB, IHC(P) and ELISA
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MHC class I Antibody (W6/32) for WB applications. These reagents are now offered in bundles with MHC class I Antibody (W6/32) (see ordering information below).
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MHC class I Antibody (W6/32) is a mouse monoclonal IgG2a kappa light chain antibody that specifically detects HLA-A, HLA-B, and HLA-C molecules of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). MHC class I monoclonal antibody (W6/32) recognizes a monomorphic epitope present on the heavy chain of human MHC class I molecules, enabling broad detection of these essential cell surface proteins. MHC class I molecules, also known as human leukocyte antigens (HLA) class I, including HLA-A, HLA-B, and HLA-C, are crucial for the immune system′s ability to present endogenously derived peptide antigens to cytotoxic T lymphocytes (CD8+ T cells). By presenting peptides typically 8-10 amino acids in length, MHC class I molecules allow the immune system to monitor cellular health and identify infected or malignant cells. MHC class I molecules function is vital for immune surveillance and initiating cytotoxic immune responses, playing a key role in controlling viral infections and tumor development. Each MHC class I molecule is composed of a heavy alpha chain and a light chain called β2-microglobulin. The heavy alpha chain contains a peptide-binding groove that is essential for antigen presentation to cytotoxic T lymphocytes. The light chain, β2-microglobulin, is non-covalently associated with the alpha chain and is crucial for the proper folding, stability, and surface expression of the MHC class I complex. Dysregulation or downregulation of MHC class I expression can lead to immune evasion by pathogens or cancer cells, highlighting their importance in disease processes. MHC class I (W6/32) antibody serves as a valuable tool for researchers studying antigen presentation, immune recognition, and related immunological functions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MHC class I Antibody (W6/32) References:
- Polymorphism and evolution of HLA class I and II genes and molecules. | Little, AM. and Parham, P. 1999. Rev Immunogenet. 1: 105-23. PMID: 11256568
- Accessory proteins that control the assembly of MHC molecules with peptides. | Van Kaer, L. 2001. Immunol Res. 23: 205-14. PMID: 11444385
- The major histocompatibility complex of the rat (Rattus norvegicus). | Günther, E. and Walter, L. 2001. Immunogenetics. 53: 520-42. PMID: 11685465
- Molecular genetics of the HLA complex. | Fischer, GF. and Mayr, WR. 2001. Wien Klin Wochenschr. 113: 814-24. PMID: 11732117
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MHC class I Antibody (W6/32) | sc-32235 | 200 µg/ml | $322.00 | |||
MHC class I Antibody (W6/32): m-IgG Fc BP-HRP Bundle | sc-528444 | 200 µg Ab; 10 µg BP | $361.00 | |||
MHC class I Antibody (W6/32): m-IgGκ BP-HRP Bundle | sc-520822 | 200 µg Ab, 40 µg BP | $361.00 | |||
MHC class I Antibody (W6/32): m-IgG2a BP-HRP Bundle | sc-547101 | 200 µg Ab; 10 µg BP | $361.00 | |||
MHC class I Antibody (W6/32) AC | sc-32235 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MHC class I Antibody (W6/32) HRP | sc-32235 HRP | 200 µg/ml | $322.00 | |||
MHC class I Antibody (W6/32) FITC | sc-32235 FITC | 200 µg/ml | $336.00 | |||
MHC class I Antibody (W6/32) PE | sc-32235 PE | 200 µg/ml | $349.00 | |||
MHC class I Antibody (W6/32) Alexa Fluor® 488 | sc-32235 AF488 | 200 µg/ml | $364.00 | |||
MHC class I Antibody (W6/32) Alexa Fluor® 546 | sc-32235 AF546 | 200 µg/ml | $364.00 | |||
MHC class I Antibody (W6/32) Alexa Fluor® 594 | sc-32235 AF594 | 200 µg/ml | $364.00 | |||
MHC class I Antibody (W6/32) Alexa Fluor® 647 | sc-32235 AF647 | 200 µg/ml | $364.00 | |||
MHC class I Antibody (W6/32) Alexa Fluor® 680 | sc-32235 AF680 | 200 µg/ml | $364.00 | |||
MHC class I Antibody (W6/32) Alexa Fluor® 790 | sc-32235 AF790 | 200 µg/ml | $364.00 | |||
MHC class I Antibody (W6/32) B | sc-32235 B | 200 µg/ml | $326.00 |