epitope mapping near the C-terminus of EPAS-1 of human origin
recommended for detection of EPAS-1 of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
blocking peptide, sc-32147 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-32147 X, 200 µg/0.1 ml
EPAS-1 Background Information Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1å, HIF-1∫ (also designated Arnt 1), EPAS-1 (also designated HIF-2å) and HIF-3å, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis (1-4). Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family (1,2). In response to hypoxia, HIF-1å is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex (4). The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription (4). Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1å dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1å independent (1,5). EPAS-1 and HIF-3å have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia (2,6).
