epitope mapping near the C-terminus of C3G of human origin
recommended for detection of C3G of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
C3G Background Information Ras p21 is the prototype of a superfamily of GTPases that is involved in the regulation of a wide variety of cellular processes. Ras signals in its GTP-bound form but is “turned off” when bound to GDP. When unregulated or constitutively turned on by mutations, Ras signaling contributes to malignant transformation. The switch between active and inactive Ras is controlled by GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs). C3G was isolated in a screen for proteins that could bind the SH3 domain of the Crk proto-oncogene product. The carboxy-terminus of the C3G protein displays significant sequence similarity to Ras-GRF/Cdc25Mm and mSos and can substitute for Cdc25 function in S. cerevisiae. These observations strongly suggest that C3G is a GEF for Ras and is involved in the regulation of Ras signaling through Crk. The C3G gene maps to human chromosome 9q34.3 in proximity to the gene that encodes c-Abl, a proto-oncogene that regulates Crk.
C3G (L-19)
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C3G (L-19): sc-32055. Western blot analysis of C3G expression in non-transfected: sc-117752 (A) and human C3G transfected: sc-115806 (B) 293T whole cell lysates.
