Rad50 Background Information Multiple pathways promote short-sequence recombination (SSR) in Saccharomyces cerevisiae. When gene conversion is initiated by a double-strand break (DSB), any nonhomologous DNA that may be present at the ends must be removed before new DNA synthesis can be initiated. Removal of a 3' nonhomologous tail in S. cerevisiae depends on the nucleotide excision repair endonuclease Rad1/Rad10, and also on the mismatch repair proteins Msh2 and Msh3. Also important for SSR is the MRE11 complex (also known as M/R/X), which is a multisubunit nuclease composed of MRE11, Rad50 and Nbs1/Xrs2. Genetic evidence suggests that Rad1/10 and M/R/X act on the same class of substrates during SSR. The MRE11 complex plays a central role in chromosomal maintenance and functions in homologous recombination, telomere maintenance and sister chromatid association. Mutations in the genes that encode components of the MRE11 complex result in DNA-damage sensitivity, genomic instability, telomere shortening and aberrant meiosis. Specifically, Rad50 contains a zinc-hook structure involved in joining MRE11 complexes in DNA recombination and repair.
Rad50 (yQ-20)
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Rad50 (yQ-20): sc-30566. Western blot analysis of Rad50 expression in K-562 whole cell lysate.
