epitope mapping at the C-terminus of RNase H1 of human origin
recommended for detection of RNase H1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
RNase H1 Background Information The human RNase H1 enzyme is a cytoplasmic endonuclease that degrades the RNA of RNA-DNA hybrids resulting in 5'-phosphomonoester products. Mn2+ and N-ethylmaleimide can inhibit Mg2+-dependent RNase H1 activity. The RNase H1 gene is present at similar levels in all human cells and tissues, indicating that RNase H1 may be a housekeeping protein. The human RNase H1 gene maps to chromosome 2p25.3 with pseudogenes present on chromosome 17p11.2 and chromosome 1q.
RNase H1 (C-18)
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RNase H1 (C-18): sc-30319. Western blot analysis of RNase H1 expression in non-transfected: sc-117752 (A) and mouse RNase H1 transfected: sc-123223 (B) 293T whole cell lysates.
RNase H1 (C-18): sc-30319. Western blot analysis of RNase H1 expression in Caki-1 whole cell lysate.