p-Flg (Tyr 653/654)-R Antibody: sc-30262-R

Flg Background Information
Acidic and basic fibroblast growth factors (FGFs) are members of a family of multifunctional polypeptide growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuro-ectodermal origin (1). Like other growth factors, FGFs act by binding and activating specific cell surface receptors. A total of four members of the FGF receptor family have been identified and cloned. These include the Flg receptor or FGFR-1 (2,3), the Bek receptor or FGFR-2 (4), FGFR-3 (5) and FGFR-4 (6). Each of these receptors consists of an extracellular ligand binding region containing three immunoglobulin-like domains (3,4), a transmembrane domain and a cytoplasmic tyrosine kinase domain (3,4). In addition to multiple receptors for the FGF family, variant forms of Flg and Bek have been described that probably arise from alternative splicing, thereby increasing the complexity of the FGF receptor family (7). The binding of FGF to Flg leads to the autophosphorylation of several tyrosine residues on Flg. (8). Proper phosphorylation of Tyr 766 is essential for interaction with PLCg and subsequently, phosphatidylinositol hydrolysis and the release of calcium from internal stores (8). Phospho-FGF receptor (Tyr653/654) was detected in embryonic livers only, indicating a physiological role of FGF during early liver development.