TIM (FL-249) Antibody: sc-30145

TIM Background Information
Glycolysis is an evolutionarily conserved series of ten chemical reactions that utilizes eleven enzymes to concomitantly generate pyruvate and ATP from glucose. Triosephosphate isomerase, known as TIM or TPI, is ubiquitously expressed and catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate in the glycolitic pathway. The human TIM gene spans 3.5 kilobase pairs, contains seven exons and encodes a 249 amino acid protein. The TIM promoter element contains a TATA box (positions -27 to -21) and multiple GC boxes (positions -126 to -48) that variably conform to the consensus Sp1-binding site. The GC boxes function in cis to the TATA box to control both the frequency and position of transcription initiation. Deficiencies in TIM result in a rare autosomal recessive condition where a metabolic block in glycolysis and accumulating DHAP in erythrocytes can lead to non-spherocytic hemolytic anemia, recurrent infections, cardiomyopathy, and neuromuscular dysfunctions.