MES solution (CAS 4432-31-9)

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Synonym:	2-(N-Morpholino)ethanesulfonic acid
Application:	Used as a running buffer for resolving very small proteins
CAS Number:	4432-31-9
Molecular Weight:	195.24
Molecular Formula:	C₆H₁₃NO₄S

Description

MES solution [2-(N-Morpholino) ethanesulfonic Acid] has a pKa value near physiological pH, it is not absorbed through cell membranes, and it is essentially transparent to UV light. It is primarily used as a running buffer for resolving very small proteins (ex: 13 kDa) on bis-tris gels. Also used in cation-exchange chromatography and as a biological buffer in plant cell cultures. MES solutions can be prepared to the desired pH by titrating the free acid with sodium hydroxide.

Also available: MES hydrate (sc-216094); MES, Sodium Salt (sc-216095); MES hemisodium salt (sc-216093)

Technical Information

Formulation:	0.5 M in H2O
Physical State:	Liquid
pH:	2.5-4.0 (25 °C in neat)
Solubility:	Soluble in water (miscible).
Refractive Index:	n²⁰_D 1.52 (Predicted)

Safety and Reference Information

WGK Germany:	1
PubChem CID:	78165
Merck Index:	14: 5902
EC Number:	224-632-3
Beilstein Registry:	6350956
SMILES:	C1COCCN1CCS(=O)(=O)O

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Graf, H., et al. 1994. Bioseparation. 4: 7-20. PMID: 7764588
3. Anfossi, L., et al. 2003. Ann Chim. 93: 499-512. PMID: 12911143
4. Mönch, S., et al. 2010. Anal. Biochem. 398: 150-160. PMID: 19903444
5. Teste, B., et al. 2010. Talanta. 81: 1703-1710. PMID: 20441961

SDS

Datasheet/SDS