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Amylase siRNA (h), shRNA and Lentiviral Particle Gene Silencers

See Amylase Antibodies for analysis of cellular responses to Amylase gene silencers, including Amylase siRNA, Amylase shRNA Plasmids and Amylase shRNA Lentiviral Particles.
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
Amylase siRNA (h) sc-29675 10 µM $258
Amylase (h)-PR sc-29675-PR 10 µM, 20 µl $23
Amylase shRNA Plasmid (h) sc-29675-SH 20 µg $520
Amylase shRNA (h) Lentiviral Particles sc-29675-V 200 µl $625

Amylase siRNA (h): sc-29675

 |  siRNADatasheet / Protocol
  • 10 µM, 50-100 transfections
  • siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
  • for independent verification of Amylase gene silencing results, individual siRNA duplex components are also available upon request
  • suitable control antibody: Amylase (C-20): sc-12821
  • sqRT-PCR Primer also available: Amylase (h)-PR: sc-29675-PR, 20 µl
 
 Recommended siRNA Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
siRNA Dilution Buffer sc-29527 1.5 ml $5
siRNA Transfection Reagent sc-29528 0.3 ml $142
siRNA Transfection Medium sc-36868 20 ml $9
siRNA Reagent System sc-45064 1 kit $331
Control siRNA-A sc-37007 10 µM $77
Control siRNA (FITC Conjugate)-A sc-36869 10 µM $104
Amylase shRNA Plasmid (h): sc-29675-SH shRNA Datasheet / Protocol
  • 20 µg, up to 20 transfections
  • shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
  • shRNA sequences correspond to Amylase siRNA Gene Silencer sequences
  • provided as transfection-ready purified plasmid DNA target-specific gene silencing
  • after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
  • shRNA Plasmids are intended only for non-commercial use. Click here for details.
  • suitable control antibody: Amylase (C-20): sc-12821
  • sqRT-PCR Primer also available: Amylase (h)-PR: sc-29675-PR, 20 µl
 
Amylase shRNA (h)
Lentiviral Particles: sc-29675-V 		Lentiviral Particles Datasheet / Protocol
  • 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
  • Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
  • Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
  • shRNA sequences correspond to Amylase siRNA siRNA Gene Silencer sequences
  • After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
  • Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
  • Lentiviral Particles are intended for non-commercial use. Click here for details.
 Recommended Lentiviral Particle Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Control shRNA Lentiviral Particles-A sc-108080 200 µl $176
copGFP Control Lentiviral Particles sc-108084 200 µl $176
Puromycin dihydrochloride sc-108071 25 mg $30
Polybrene® sc-134220 10 mg/ml $18
 Recommended Control Antibody (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Amylase Antibody (C-20) sc-12821 200 µg/ml $279
donkey anti-goat IgG-HRP sc-2020 200 µg/0.5 ml $70
 
Recommended Support Products:
(click button of application of choice)
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human AMY1A 276 1p21.1 NM_001008221, NM_004038 P04745
 104700
Human AMY1B 277 1p21.1 NM_001008218 P04745
 104701
Human AMY1C 278 1p21.1 NM_001008219 NP_001008220
 104702
Human AMY2A 279 1p21.1 NM_000699 P04746
 104650
Human AMY2B 280 1p21.1 NM_020978 P19961
 104660
 
 Recommended shRNA Support Reagents (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
shRNA Plasmid Transfection Reagent sc-108061 0.2 ml $71
shRNA Plasmid Transfection Medium sc-108062 20 ml $8
Control shRNA Plasmid-A sc-108060 20 µg $83
Control shRNA Plasmid-B sc-108065 20 µg $83
Control shRNA Plasmid-C sc-108066 20 µg $83
copGFP Control Plasmid sc-108083 20 µg $83
Puromycin dihydrochloride sc-108071 25 mg $30
Amylase siRNA (h) Data
Click on image to enlarge
sc-29675
Amylase shRNA Plasmid (h): sc-29675-SH. Western blot analysis of human Amylase expression in Amylase transfected (A) and Amylase/Amylase shRNA Plasmid cotransfected (B) 293T cells. Blot probed with Amylase (C-20): sc-12821. Actin (I-19): sc-1616 used as specificity and loading control.
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Datasheets: siRNA, shRNA, Lentiviral Particles
Protocols: siRNA, shRNA, Lentiviral Particles
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