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Fluorescein Diacetate (CAS 596-09-8)

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Synonym:Cellstain-FDA
Application:A useful live-cell fluorescent stain
CAS Number:596-09-8
Purity:97%
Molecular Weight:416.4
Molecular Formula:C24H16O7
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
Fluorescein Diacetate sc-294598 5 g $75
Description
Fluorescein diacetate stains living cells green, and is a substrate for esterases. Fluorescein Diacetate is suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.
Technical Information
Physical State:Solid
Solubility:Soluble in chloroform, DMSO, ethanol, and water.
Storage:Store at room temperature
Melting Point:200-203 ºC
Boiling Point:604.70 °C at 760 mmHg (Predicted)
Density:1.47 g/cm3 (Predicted)
Refractive Index:n20D 1.68 (Predicted)
Fluoresence:λex 495 nm, λem 520 nm
IC50:HEK293 : IC50 = >89.2 µM (human); Beta Lactamase: IC50 = 39.8 µM (Pseudomonas aeruginosa); HIV-1(RF) : IC50 = 43.4 µM (CEM); HIV-1(RF) : EC5050 = >200 µM (CEM); HEK293 : EC5050 = >54.7 µM (human); Insulin-degrading enzyme: EC5050 = >54.7 µM (human)
Safety and Reference Information
WGK Germany:3
PubChem CID:65047
MDL Number:MFCD00005062
EC Number:209-877-6
Beilstein Registry:62575
SMILES:CC(=O)Oc1ccc2c(c1)Oc1cc(ccc1C12OC(=O)c2ccccc12)OC(=O)C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
References
Clements, K.M., et al. 2001. Osteoarthritis Cartilage 9, 499. Bedner, E., et al. 1998. Cytometry 33, 1. Guilbault, G.G., and Kramer, D.N. 1964. Anal. Chem. 36, 409.
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