Citrate Concentrated Solution (CAS 68-04-2)

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Application:	1M; may be used to prepare citrate buffer for antigen unmasking in IHC applications
CAS Number:	68-04-2
Molecular Weight:	258.07
Molecular Formula:	C₆H₅Na₃O₇

Description

Citrate Concentrated Solution is 1 M sodium citrate in H₂O. As a conjugate base of a weak acid, sodium citrate can perform as a biological buffering agent because it resists changes in pH. May be used to prepare sodium citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links; thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity; as a calcium chelator, it forms complexes that disrupt the tendency of blood to clot.
Additional forms available:
Citric Acid Monohydrate (sc-203327)
Citric Acid, Anhydrous (sc-211113)
Sodium Citrate, Dihydrate (sc-203383)
Citric Acid Trisodium Salt (sc-214745)
Sodium citrate monobasic (sc-215869)
Sodium citrate tribasic hydrate (sc-236898)

Usage

For antigen retrieval, prepare a solution of 10 mM sodium citrate buffer, pH 6.0.

Technical Information

Formulation:	1 M
Appearance:	Clear liquid
Physical State:	Liquid
Storage:	Store at room temperature
Density:	1.14 g/mL

Safety and Reference Information

WGK Germany:	1
PubChem CID:	6224
Merck Index:	14: 8602
MDL Number:	MFCD00012462
EC Number:	200–675–3
SMILES:	C(C(=O)[O-])C(CC(=O)[O-])(C(=O)[O-])O.[Na+].[Na+].[Na+]

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

1. Vandemark, P.J. and Smith, P.F. 1964. J. Bacteriol. 88: 1602-1607. PMID: 14240945
2. Shi, S.R., et al. 1993. J. Histochem. Cytochem. 41: 1599-1604. PMID: 7691930
3. Echezarreta, G., et al. 1997. Sangre. 42: 369-375. PMID: 9424736
4. Uszyński, W., et al. 2007. Folia Histochem. Cytobiol. 45: 33-36. PMID: 17378243

SDS

Datasheet/SDS