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FAS siRNA, shRNA and Lentiviral Particle Gene Silencers
- 10 µM, 50-100 transfections
- siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
- for independent verification of FAS gene silencing results, individual siRNA duplex components are also available upon request
- suitable control antibody: FAS (A-20): sc-1023
- sqRT-PCR Primer also available: FAS (h)-PR: sc-29311-PR, 20 µl
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- 20 µg, up to 20 transfections
- shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
- shRNA sequences correspond to FAS siRNA Gene Silencer sequences
- provided as transfection-ready purified plasmid DNA target-specific gene silencing
- after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
- shRNA Plasmids are intended only for non-commercial use. Click here for details.
- suitable control antibody: FAS (A-20): sc-1023
- sqRT-PCR Primer also available: FAS (h)-PR: sc-29311-PR, 20 µl
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- 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
- Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
- Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
- shRNA sequences correspond to FAS siRNA siRNA Gene Silencer sequences
- After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
- Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
- Lentiviral Particles are intended for non-commercial use. Click here for details.
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| FAS siRNA (h) Product Citations |
See how others have used FAS siRNA (h): sc-29311.
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FAS siRNA (h)
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FAS siRNA (h): sc-29311. Western blot analysis of FAS expression in non-transfected control (A) and FAS siRNA transfected (B) HeLa cells. Blot probed with FAS (B-10): sc-8009. Actin (I-19): sc-1616 used as specificity and loading control.
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