shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
shRNA sequences correspond to ERK 1 siRNA Gene Silencer sequences
provided as transfection-ready purified plasmid DNA target-specific gene silencing
after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
See how others have used ERK 1 siRNA (m): sc-29308.
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ERK 1 siRNA (m)
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ERK 1 siRNA (m): sc-29308. Western blot analysis of ERK 1 expression in non-transfected control (A) and ERK 1 siRNA transfected (B) NIH/3T3 cells. Blot probed with ERK 1 (C-16): sc-93. Actin (C-2): sc-8432 used as specificity and loading control.