TLR4 Antibody (25): sc-293072

TLR4 Antibody (25) is a mouse monoclonal IgG1 kappa light chain antibody that detects TLR4 protein of mouse, rat, human, and canine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). anti-TLR4 antibody (25) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). TLR4 plays a crucial role in the immune system as a pattern recognition receptor that identifies pathogen-associated molecular patterns, particularly lipopolysaccharides from Gram-negative bacteria. This recognition is vital for initiating innate immune responses, leading to the activation of signaling pathways that result in the production of pro-inflammatory cytokines and the recruitment of immune cells to sites of infection. TLR4 is primarily located on the cell surface of immune cells such as macrophages and dendritic cells, where TLR4 acts as a sentinel for microbial invasion. Proper functioning of TLR4 is essential for maintaining immune homeostasis and responding effectively to infections. Dysregulation of TLR4 signaling has been implicated in various inflammatory diseases, making TLR4 a significant target for therapeutic intervention. When utilized in immunostaining applications, TLR4 monoclonal antibody (25) demonstrates both nuclear and cytoplasmic localization, highlighting TLR4′s dynamic role in cellular signaling. Expression of TLR4 is notably elevated in peripheral blood leukocytes, macrophages, and monocytes, underscoring TLR4′s importance in the immune response across multiple species.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TLR4 Antibody (25) References:

1. Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction.  |  Chow, JC., et al. 1999. J Biol Chem. 274: 10689-92. PMID: 10196138
2. TLR6: A novel member of an expanding toll-like receptor family.  |  Takeuchi, O., et al. 1999. Gene. 231: 59-65. PMID: 10231569
3. Peptidoglycan- and lipoteichoic acid-induced cell activation is mediated by toll-like receptor 2.  |  Schwandner, R., et al. 1999. J Biol Chem. 274: 17406-9. PMID: 10364168
4. Host defense mechanisms triggered by microbial lipoproteins through toll-like receptors.  |  Brightbill, HD., et al. 1999. Science. 285: 732-6. PMID: 10426995
5. Drosophila Toll and IL-1 receptor.  |  Gay, NJ. and Keith, FJ. 1991. Nature. 351: 355-6. PMID: 1851964
6. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity.  |  Medzhitov, R., et al. 1997. Nature. 388: 394-7. PMID: 9237759
7. A family of human receptors structurally related to Drosophila Toll.  |  Rock, FL., et al. 1998. Proc Natl Acad Sci U S A. 95: 588-93. PMID: 9435236
8. Toll-like receptor-2 mediates lipopolysaccharide-induced cellular signalling.  |  Yang, RB., et al. 1998. Nature. 395: 284-8. PMID: 9751057

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TLR4 Antibody (25) has 704 citations in a variety of scientific publications.

Primary antibodies like Anti-TLR4 Antibody (25) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TLR4 Antibody (25) Specifications

Alternate Product Name: TLR4

Product Clone Name: 25

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 198-395

Epitope Target: TLR4

Epitope Species: mouse

Product Reactivity/Detection: TLR4

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 704 citations in various scientific publications

Available Conjugates/Forms for TLR4 Antibody (25): AC: Agarose, HRP: Horseradish Peroxidase

The preferred TLR4 antibody is TLR4 Antibody (HTA125): sc-13593.