shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
shRNA sequences correspond to c-Src siRNA Gene Silencer sequences
provided as transfection-ready purified plasmid DNA target-specific gene silencing
after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
shRNA sequences correspond to c-Src siRNA siRNA Gene Silencer sequences
After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
See how others have used c-Src siRNA (h): sc-29228.
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c-Src siRNA (h)
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c-Src siRNA (h): sc-29228. Western blot analysis of c-Src expression in non-transfected control (A) and c-Src siRNA transfected (B) HeLa cells. Blot probed with c-Src (B-12): sc-8056. GAPDH (FL-335): sc-25778 used as specificity and loading control.
c-Src siRNA (h): sc-29228. Immunofluorescence staining of methanol-fixed, control HeLa (A) and c-Src siRNA silenced HeLa (B) cells showing diminished membrane staining in the siRNA silenced cells. Cells probed with c-Src (SRC 2): sc-18.
