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BRCA1 siRNA, shRNA and Lentiviral Particle Gene Silencers
- 10 µM, 50-100 transfections
- siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
- for independent verification of BRCA1 gene silencing results, individual siRNA duplex components are also available upon request
- suitable control antibody: BRCA1 (C-20): sc-642
- sqRT-PCR Primer also available: BRCA1 (h)-PR: sc-29219-PR, 20 µl
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- 20 µg, up to 20 transfections
- shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
- shRNA sequences correspond to BRCA1 siRNA Gene Silencer sequences
- provided as transfection-ready purified plasmid DNA target-specific gene silencing
- after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
- shRNA Plasmids are intended only for non-commercial use. Click here for details.
- suitable control antibody: BRCA1 (C-20): sc-642
- sqRT-PCR Primer also available: BRCA1 (h)-PR: sc-29219-PR, 20 µl
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- 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
- Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
- Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
- shRNA sequences correspond to BRCA1 siRNA siRNA Gene Silencer sequences
- After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
- Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
- Lentiviral Particles are intended for non-commercial use. Click here for details.
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Product Citations
Recommended Support Products:
(click button of application of choice)
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
BRCA1 |
672 |
17q21.31 |
NM_007294, NM_007295, NM_007296, NM_007297, NM_007298, NM_007299, NM_007300, NM_007302, NM_007303, NM_007304, NM_007305 |
P38398
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113705 |
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| BRCA1 siRNA (h) Product Citations |
See how others have used BRCA1 siRNA (h): sc-29219.
4 total citations Loading citations.
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BRCA1 siRNA (h)
Click on image to enlarge
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BRCA1 siRNA (h): sc-29219. Western blot analysis of BRCA1 expression in non-transfected control (A) and BRCA1 siRNA transfected (B) UV-treated HeLa cells. Blot probed with BRCA1 (D-9): sc-6954. γ Tubulin (C-11): sc-17787 used as specificity and loading control. BRCA1 siRNA (h): sc-29219. Immunofluorescence staining of methanol-fixed, control HeLa (A) and BRCA1 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with BRCA1 (I-20): sc-646. BRCA1 siRNA (h): sc-29219. Western blot analysis of BRCA1 expression in non-transfected control (A) and BRCA1 siRNA transfected (B) A-431 cells. Blot probed with BRCA1 (C-20): sc-642. α-actinin (H-2): sc-17829 used as specificity and loading control. |
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